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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Con A activated canine peripheral blood cells.
- 亚型:
IgG2a
- 形态:
Purified IgG conjugated to StarBright Violet 570 - liquid
- 克隆性:
Monoclonal Antibody
- 标记物:
StarBright Violet 570
- 适应物种:
Dog
- 保质期:
自发货之日起 12 个月
- 目录编号:
YKIX334.2
- 供应商:
伯乐生命医学产品(上海)有限公司
- 应用范围:
F
- 浓度:
For information on the concentration of our StarBright Dye conjugated reagents please visit our FAQ page.
- 抗体名:
MHC Class II Monomorphic antibody | YKIX334.2
- 规格:
100 Tests/0.5ml
Rat anti Dog MHC Class II Monomorphic antibody, clone YKIX334.2 recognizes a monomorphic epitope on canine MHC Class II and was classified at the First Canine Leucocyte Antigen Workshop (Cobbold et al. 1994).The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In dogs, this is referred to as the dog leukocyte antigen (DLA) region. Rat anti Dog MHC Class II immunoprecipitates an antigen of ~32/34 kDa and blocks the proliferation of MHC Class II dependent responses in vitro. In dogs, MHC Class II is expressed by all peripheral blood mononuclear cells.
储存信息
This product is shipped at ambient temperature.
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ | Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 5μl of the suggested working dilution to label 106 cells in 100μl. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
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文献和实验该产品被引用文献
References for MHC Class II Monomorphic antibody
- Cobbold, S. & Metcalfe, S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW).
Tissue Antigens. 43 (3): 137-54. - Watson, C.J. et al. (1994) Immunosuppression of canine renal allograft recipients by CD4 and CD8 monoclonal antibodies.
Tissue Antigens. 43 (3): 155-62. - Liu, Y. et al. (2000) Immunosuppressant-free allotransplantation of the tracheaThe antigenicity of tracheal grafts can be reduced by removing the epithelium and mixed glands from the graft by detergent treatment.
J Thorac Cardiovasc Surg. 120: 108-14. - Sanchez, M.A. et al. (2004) Organ-specific immunity in canine visceral leishmaniasis: analysis of symptomatic and asymptomatic dogs naturally infected with Leishmania chagasi.
Am J Trop Med Hyg. 70: 618-24. - Reis, A.B. et al. (2006) Phenotypic features of circulating leucocytes as immunological markers for clinical status and bone marrow parasite density in dogs naturally infected by Leishmania chagasi.
Clin Exp Immunol.146: 303-11. - Bonnefont-Rebeix, C. et al. (2007) Toll-like receptor 3 (TLR3): a new marker of canine monocytes-derived dendritic cells (cMo-DC).
Vet Immunol Immunopathol. 2007 Jul 15;118(1-2):134-9. - Schütze, N. et al. (2009) Inactivated parapoxvirus ovis activates canine blood phagocytes and T lymphocytes.
Vet Microbiol. 137: 260-7. - Bund, D. et al. (2010) Canine-DCs using different serum-free methods as an approach to provide an animal-model for immunotherapeutic strategies.
Cell Immunol. 263: 88-98.
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Rat anti Dog MHC Class II Monomorphic:StarBright Violet 570
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