- 询价
- BIO-RAD
- MCA2389SBUV510
- 2025年12月11日
- F
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Balb/c macrophage precursor cell hybrids.
- 亚型：
IgG2a
- 形态：
Purified IgG conjugated to StarBright UltraViolet 510 - liquid
- 克隆性：
Monoclonal Antibody
- 标记物：
StarBright UltraViolet 510
- 适应物种：
Mouse
- 保质期：
自发货之日起 12 个月
- 目录编号：
ER-MP20
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
F
- 浓度：
For information on the concentration of our StarBright Dye conjugated reagents please visit our FAQ page.
- 抗体名：
Ly-6C antibody | ER-MP20
- 规格：
100 Tests/0.5ml
Rat anti Mouse Ly-6C antibody, clone ER-MP20 recognizes murine Ly-6C, a 131 amino acid ~14 kDa differentiation antigen, expressed on macrophage/dendritic cell precursors in mid-stage development (late CFU-M, monoblasts and immature monocytes), granulocytes, and on a wide range of endothelial cells and subpopulations of B- and T-lymphocytes.
Rat anti Mouse Ly-6C antibody, clone ER-MP20 is able to distinguish multiple mouse blood monocyte subsets: immature Ly-6Chi monocytes are recruited to acute peripheral inflammation and develop into Ly-6C+ exudate macrophages, whereas more mature Ly-6C-/lo monocytes are precursors for tissue macrophages and dendritic cells in steady state.
Rat anti Mouse Ly-6C, clone ER-MP20 can be used in conjunction with clone ER-MP12 in two colour flow cytometric analysis, to identify different stages of myeloid progenitor cells in mouse bone marrow (Leenen et al. 1990).
Rat anti Mouse Ly-6C was originally described as recognizing a protein encoded by the LY6C gene. It has subsequently become apparent that the LY6C locus demonstrates polymorphism and the LY6C gene has been re-designated LY6C2. The LY6C1 gene encodes a similar protein with ~95% sequence homology to LY6C2.
Rat anti Mouse Ly-6C antibody, clone ER-MP20 is able to distinguish multiple mouse blood monocyte subsets: immature Ly-6Chi monocytes are recruited to acute peripheral inflammation and develop into Ly-6C+ exudate macrophages, whereas more mature Ly-6C-/lo monocytes are precursors for tissue macrophages and dendritic cells in steady state.
Rat anti Mouse Ly-6C, clone ER-MP20 can be used in conjunction with clone ER-MP12 in two colour flow cytometric analysis, to identify different stages of myeloid progenitor cells in mouse bone marrow (Leenen et al. 1990).
Rat anti Mouse Ly-6C was originally described as recognizing a protein encoded by the LY6C gene. It has subsequently become apparent that the LY6C locus demonstrates polymorphism and the LY6C gene has been re-designated LY6C2. The LY6C1 gene encodes a similar protein with ~95% sequence homology to LY6C2.
储存信息
This product is shipped at ambient temperature.
Store at +4°C.
DO NOT FREEZE.
This product should be stored undiluted.
Store at +4°C.
DO NOT FREEZE.
This product should be stored undiluted.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ | Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 5μl of the suggested working dilution to label 106 cells in 100μl. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
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文献和实验该产品被引用文献
References for Ly-6C antibody
- Zhang, Y. & Bliska, J.B. (2010) YopJ-promoted cytotoxicity and systemic colonization are associated with high levels of murine interleukin-18, gamma interferon, and neutrophils in a live vaccine model of Yersinia pseudotuberculosis infection.
Infect Immun 78: 2329-41. - Leenen, P.J. et al. (1990) Murine macrophage precursor characterization. II. Monoclonal antibodies against macrophage precursor antigens.
Eur J Immunol. 20 (1): 27-34. - de Bruijn, M.F. et al. (1998) Bone marrow cellular composition in Listeria monocytogenes infected mice detected using ER-MP12 and ER-MP20 antibodies: a flow cytometric alternative to differential counting.
J Immunol Methods. 217 (1-2): 27-39. - Schatteman, G.C. et al. (2010) Lin- Cells Mediate Tissue Repair by Regulating MCP-1/CCL-2.
Am J Pathol. 177: 2002-10. - Baumeister, T. et al. (2003) Interleukin-3Ralpha+ myeloid dendritic cells and mast cells develop simultaneously from different bone marrow precursors in cultures with interleukin-3.
J Invest Dermatol. 121: 280-8. - Devey, L. et al. (2009) Tissue-resident macrophages protect the liver from ischemia reperfusion injury via a heme oxygenase-1-dependent mechanism.
Mol Ther. 17: 65-72. - Nikolic, T. et al. (2003) Developmental stages of myeloid dendritic cells in mouse bone marrow.
Int Immunol. 15: 515-24. - Wynn, A.A. et al. (2001) Role of granulocyte/macrophage colony-stimulating factor in zymocel-induced hepatic granuloma formation.
Am J Pathol. 158 (1): 131-45.
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Rat anti Mouse Ly-6C:StarBright UltraViolet 510
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