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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Purified human tonsil lymphocyte membranes.
- 亚型:
IgG2a
- 形态:
Purified IgG conjugated to StarBright UltraViolet 400 - liquid
- 克隆性:
Monoclonal Antibody
- 标记物:
StarBright UltraViolet 400
- 适应物种:
Human
- 保质期:
自发货之日起 12 个月
- 目录编号:
W6/32
- 供应商:
伯乐生命医学产品(上海)有限公司
- 应用范围:
F
- 浓度:
For information on the concentration of our StarBright Dye conjugated reagents please visit our FAQ page.
- 抗体名:
HLA ABC antibody | W6/32
- 规格:
100 Tests/0.5ml
Mouse anti Human HLA ABC antibody, clone W6/32 recognizes an antigenic determinant shared among products of the HLA A, B and C loci. Clone W6/32 recognizes a conformational epitope, reacting with HLA class I alpha3 and alpha2 domains. The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class I proteins encoded by the HLA which are HLA A, HLA B and HLA C. These proteins are found on the surface of almost all nucleated somatic cells.
Mouse anti Human HLA ABC antibody, clone W6/32 is routinely tested in flow cytometry on human peripheral blood lymphocytes.
Mouse anti Human HLA ABC antibody, clone W6/32 is routinely tested in flow cytometry on human peripheral blood lymphocytes.
储存信息
This product is shipped at ambient temperature.
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ | Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 5μl of the suggested working dilution to label 106 cells in 100μl. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
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文献和实验该产品被引用文献
Source Reference
- Barnstable, C.J. et al. (1978) Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens-new tools for genetic analysis.
Cell. 14 (1): 9-20.
References for HLA ABC antibody
- Brodsky, F.M. & Parham, P. (1982) Evolution of HLA antigenic determinants: species cross-reactions of monoclonal antibodies.
Immunogenetics. 15 (2): 151-66. - Neefjes, J.J. et al. (1986) A biochemical characterization of feline MHC products: unusually high expression of class II antigens on peripheral blood lymphocytes.
Immunogenetics. 23 (5): 341-7. - Stern, P.L. et al. (1987) Class I-like MHC molecules expressed by baboon placental syncytiotrophoblast.
J Immunol. 138 (4): 1088-91. - Jacobsen, C.N. et al. (1993) Reactivities of 20 anti-human monoclonal antibodies with leucocytes from ten different animal species.
Vet Immunol Immunopathol. 39 (4): 461-6. - Verbeek, M.M. et al. (1995) T lymphocyte adhesion to human brain pericytes is mediated via very late antigen-4/vascular cell adhesion molecule-1 interactions.
J Immunol. 154 (11): 5876-84. - Raftery, M.J. et al. (2002) Hantavirus infection of dendritic cells.
J Virol. 76: 10724-33. - Dressel, R. et al. (2003) Differential effect of acute and permanent heat shock protein 70 overexpression in tumor cells on lysability by cytotoxic T lymphocytes.
Cancer Res. 63 (23): 8212-20. - Ishitani, A. et al. (2003) Protein expression and peptide binding suggest unique and interacting functional roles for HLA-E, F, and G in maternal-placental immune recognition.
J Immunol. 171 (3): 1376-84.
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Mouse anti Human HLA ABC:StarBright UltraViolet 400
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