- 询价
- BIO-RAD
- MCA1031SBR670
- 2025年12月19日
- F
- Mouse
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Mouse spleen cells.
- 亚型：
IgG2b
- 形态：
Purified IgG conjugated to StarBright Red 670 - liquid
- 克隆性：
Monoclonal Antibody
- 标记物：
StarBright Red 670
- 适应物种：
Mouse
- 保质期：
自发货之日起 12 个月
- 目录编号：
YW62.3
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
F
- 浓度：
For information on the concentration of our StarBright Dye conjugated reagents please visit our FAQ page.
- 抗体名：
CD45 antibody | YW62.3
- 规格：
100 Tests/0.5ml
大鼠抗小鼠CD45抗体（克隆号YW62.3） 能够识别小鼠CD45细胞表面抗原，该抗原是一种单次跨膜的I型糖蛋白，也称为蛋白酪氨酸磷酸酶受体C型（PTPRC），最初被命名为白细胞共同抗原（LCA）。CD45是一种在所有白细胞表面表达的180-220 kDa糖蛋白。
在小鼠中，CD45由3个等位基因编码，在不同近交系中呈现差异性表达。Ly5基因最初被定义为：C57BL/6品系表达其基因产物LY5.1，SJL品系表达Ly5.2，随后扩展出第三个编码Ly5.3的等位基因。1987年发生命名修订，C57BL/6品系的等位基因变为Ly5b（编码Ly5.2），SJL小鼠的等位基因变为Ly5a（编码Ly5.1）。1992年进一步调整命名：Ly5.1改为CD45.1（SJL），Ly5.2改为CD45.2（C57BL/6）。最终，因证实CD45抗原与受体相关蛋白酪氨酸磷酸酶具有同源性，CD45a基因更名为Ptprca，CD45b更名为Ptprcb。
小鼠白细胞表达多种CD45亚型，其差异取决于三个外显子（A、B、C）的选择性剪接模式，这些外显子编码位于CD45胞外区N端约50个氨基酸的区域。这些特定表达的蛋白统称为CD45R，其具体命名由表达的密码子产物决定。
大鼠抗小鼠CD45抗体（克隆号YW62.3）可与小鼠所有CD45亚型发生反应。
注：已观察到该抗体与人体组织存在一定的交叉反应性。
| 项目 | 说明 |
|---|---|
| 靶标物种 | 小鼠 |
| 产品形式 | 纯化IgG，偶联StarBright Red 670 - 液体 |
| 制备方式 | 采用Protein G亲和层析法从组织培养上清液中纯化IgG |
| 缓冲溶液 | 磷酸盐缓冲盐水 |
| 防腐剂/稳定剂 | 0.09% (NaN₃) 1% 牛血清白蛋白 0.1% Pluronic F68 0.1% PEG 3350 0.05% Tween 20 |
| 免疫原 | 小鼠脾细胞 |
| 近似蛋白浓度 | 关于StarBright染料偶联试剂的浓度信息，请访问常见问题页面 |
| 融合伙伴 | 免疫DA大鼠脾细胞与大鼠Y3/Ag1.2.3骨髓瘤细胞系细胞融合 |
| 最大激发/发射波长 | 见下方荧光团特性表 |
| 监管声明 | 仅用于研究目的 |
| 保质期 | 自发货之日起12个月 |
| 知识产权声明 | 本产品受美国专利号10,150,841及相关美国和外国同等专利保护 |
荧光团特性
| 荧光团 | 最大激发波长 (nm) | 最大发射波长 (nm) |
|---|---|---|
| StarBright Red 670 | 653 | 666 |
储存信息
本品在环境温度下运输。
储存于+4°C。请勿冷冻。
应储存未稀释的原液。
应用
据报道，本品适用于以下应用。此信息源自我们实验室的内部测试、同行评审的出版物或原研制作者的交流。更多细节请查阅所示参考文献。一般实验方案建议请访问抗体方案页面。
| 应用名称 | 已验证 | 最低稀释度 | 最高稀释度 |
|---|---|---|---|
| 流式细胞术 | ✓ | 原液 |
若本产品尚未针对特定技术进行测试，这并不代表其不能用于该技术。建议的工作稀释度仅供参考，建议用户使用适当的阴性/阳性对照，在自己的实验体系中对产品进行滴定以确定最佳使用浓度。
流式细胞术
建议取 5µl 推荐工作稀释度的抗体，用于标记 100µl 体积中的 10⁶ 个细胞。最佳操作流程建议：在加样前先以 6,000g 的转速离心 5 分钟。
Rat anti Mouse CD45 antibody, clone YW62.3 recognizes the murine CD45 cell surface antigen, a single pass type1 transmembrane glycoprotein also known as protein tyrosine phosphatase receptor type C (PTPRC) and originally termed Leucocyte Common Antigen (LCA). CD45 is a 180-220kDa glycoprotein expressed by all leucocytes.
CD45 is encoded by 3 alleles in mice, differentially expressed by various inbred strains. The Ly5 gene was originally described with the gene product LY5.1 expressed in C57bl/6 and Ly5.2 expressed in SJL strains (Komura et al. 1975), this was subsequently expanded to include a third allele encoding Ly5.3 (Shen et al. 1986). Further, in 1987 a reversal of nomenclature was instigated resulting in the allele in C57bl/6 becoming Ly5b encoding Ly5.2 and the allele in SJL mice becoming Ly5a encoding Ly5.1 (Morse et al. 1987). Further changes were made in 1992 with Ly5.1 becoming CD45.1 (SJL) and Ly5.2 becoming CD45.2 (C57bl/6). Finally, following work demonstrating homology between the CD45 antigen and a receptor linked protein tyrosine phosphatase the CD45a gene was renamed Ptprca and CD45b renamed Ptprcb (Charbonneau et al. 1988; Zebedee et al. 1991).
A number of different isoforms of CD45 are expressed on murine leucocytes depending on the pattern of alternative splicing of 3 exons termed A, B and C encoding regions of ~ 50 amino acids located at the N terminal region of the extracellular portion of CD45. The restricted proteins are termed CD45R with a designation depending on the expressed codon product. (Birkeland et al. 1989).
Rat anti mouse CD45 antibody, clone YW62.3 is reactive with all isoforms of murine CD45.
N.B. Some reactivity with human tissue has been observed.
储存信息
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
应用
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ | Neat |
Flow Cytometry
Use 5μl of the suggested working dilution to label 106 cells in 100μl. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验References for CD45 antibody
- Watt, S.M. et al. (1987) Cell-surface markers on haemopoietic precursors. Reagents for the isolation and analysis of progenitor cell subpopulations.
Mol Cell Probes. 1 (4): 297-326. - Kondo, Y. et al. (2007) Osteopetrotic (op/op) mice have reduced microglia, no Abeta deposition, and no changes in dopaminergic neurons.
J Neuroinflammation. 4: 31. - Wang, S. et al. (2008) Drak2 contributes to West Nile virus entry into the brain and lethal encephalitis.
J Immunol. 181: 2084-91. - Chan, D.A. et al (2009) Tumor vasculature is regulated by PHD2-mediated angiogenesis and bone marrow-derived cell recruitment.
Cancer Cell. 15: 527-38. - Lee, S. et al. (2010) CX3CR1 deficiency alters microglial activation and reduces beta-amyloid deposition in two Alzheimer's disease mouse models.
Am J Pathol. 177: 2549-62. - Dénes, A. et al. (2010) Chronic systemic infection exacerbates ischemic brain damage via a CCL5 (regulated on activation, normal T-cell expressed and secreted)-mediated proinflammatory response in mice.
J Neurosci. 30: 10086-95. - Yoshizaki, A. et al. (2010) Cell adhesion molecules regulate fibrotic process via Th1/Th2/Th17 cell balance in a bleomycin-induced scleroderma model.
J Immunol. 185: 2502-15. - Yang, J. et al. (2010) Evaluation of bone marrow- and brain-derived neural stem cells in therapy of central nervous system autoimmunity.
Am J Pathol. 177: 1989-2001.
技术资料暂无技术资料 索取技术资料
