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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Human T lymphocytes.
- 亚型:
IgG2a
- 形态:
Purified IgG conjugated to StarBright Yellow 720 - liquid
- 克隆性:
Monoclonal Antibody
- 标记物:
StarBright Yellow 720
- 适应物种:
Human
- 保质期:
自发货之日起 12 个月
- 目录编号:
F10-89-4
- 供应商:
伯乐生命医学产品(上海)有限公司
- 应用范围:
F
- 浓度:
For information on the concentration of our StarBright Dye conjugated reagents please visit our FAQ page.
- 抗体名:
CD45 antibody | F10-89-4
- 规格:
100 Tests/0.5ml
Mouse anti Human CD45 antibody, clone F10-89-4 recognizes the human CD45 cell surface antigen, also known as leucocyte common antigen (LCA). CD45 is a complex molecule existing in a number of isoforms.
Antibodies recognizing a common epitope on all of these isoforms are termed CD45 whilst those recognizing only individual isoforms are termed CD45RA or CD45RO etc.
Mouse anti Human CD45 antibody, clone F10-89-4 reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes, having a higher level of expression on lymphocytes than on granulocytes. It is routinely tested in flow cytometry on human peripheral blood leucocytes.
Mouse anti Human CD45 antibody, clone F10-89-4, has been validated for use on the Genesis Cell Isolation System with the CelSelect SlideTM technology.
Antibodies recognizing a common epitope on all of these isoforms are termed CD45 whilst those recognizing only individual isoforms are termed CD45RA or CD45RO etc.
Mouse anti Human CD45 antibody, clone F10-89-4 reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes, having a higher level of expression on lymphocytes than on granulocytes. It is routinely tested in flow cytometry on human peripheral blood leucocytes.
Mouse anti Human CD45 antibody, clone F10-89-4, has been validated for use on the Genesis Cell Isolation System with the CelSelect SlideTM technology.
储存信息
This product is shipped at ambient temperature.
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ | Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 5μl of the suggested working dilution to label 106 cells in 100μl. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
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文献和实验该产品被引用文献
Source Reference
- Dalchau, R. et al. (1980) Monoclonal antibody to a human leukocyte-specific membrane glycoprotein probably homologous to the leukocyte-common (L-C) antigen of the rat.
Eur J Immunol. 10 (10): 737-44.
References for CD45 antibody
- Quenby, S et al. (1999) Pre-implantation endometrial leukocytes in women with recurrent miscarriage.
Human Reprod. 14(9):2386-2391. - Hauser, P.V. et al. (2010) Stem cells derived from human amniotic fluid contribute to acute kidney injury recovery.
Am J Pathol. 177: 2011-21. - Mallam, E. et al. (2010) Characterization of in vitro expanded bone marrow-derived mesenchymal stem cells from patients with multiple sclerosis.
Mult Scler. 16: 909-18. - Marrinucci, D. et al. (2010) Cytomorphology of circulating colorectal tumor cells:a small case series.
J Oncol. 2010: 861341. - Paul, G. et al. (2012) The adult human brain harbors multipotent perivascular mesenchymal stem cells.
PLoS One. 7: e35577. - De Schauwer, C. et al. (2012) In search for cross-reactivity to immunophenotype equine mesenchymal stromal cells by multicolor flow cytometry.
Cytometry A. 81 (4): 312-23. - Kazane, S.A. et al. (2012) Site-specific DNA-antibody conjugates for specific and sensitive immuno-PCR.
Proc Natl Acad Sci U S A. 109: 3731-6. - Spaas, J.H. et al. (2013) Culture and characterisation of equine peripheral blood mesenchymal stromal cells.
Vet J. 195 (1): 107-13.
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Mouse anti Human CD45:StarBright Yellow 720
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