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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Human T lymphocytes.
- 亚型:
IgG2a
- 形态:
Purified IgG conjugated to StarBright Blue 700 - liquid
- 克隆性:
Monoclonal Antibody
- 标记物:
StarBright Blue 700
- 适应物种:
Human
- 保质期:
自发货之日起 12 个月
- 目录编号:
F10-89-4
- 供应商:
伯乐生命医学产品(上海)有限公司
- 应用范围:
F
- 浓度:
For information on the concentration of our StarBright Dye conjugated reagents please visit our FAQ page.
- 抗体名:
CD45 antibody | F10-89-4
- 规格:
100 Tests/0.5ml
Antibodies recognizing a common epitope on all of these isoforms are termed CD45 whilst those recognizing only individual isoforms are termed CD45RA or CD45RO etc.
Mouse anti Human CD45 antibody, clone F10-89-4 reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes, having a higher level of expression on lymphocytes than on granulocytes. It is routinely tested in flow cytometry on human peripheral blood leucocytes.
Mouse anti Human CD45 antibody, clone F10-89-4, has been validated for use on the Genesis Cell Isolation System with the CelSelect SlideTM technology.
储存信息
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
应用
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ | Neat |
Flow Cytometry
Use 5μl of the suggested working dilution to label 106 cells in 100μl. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
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文献和实验Source Reference
- Dalchau, R. et al. (1980) Monoclonal antibody to a human leukocyte-specific membrane glycoprotein probably homologous to the leukocyte-common (L-C) antigen of the rat.
Eur J Immunol. 10 (10): 737-44.
References for CD45 antibody
- Quenby, S et al. (1999) Pre-implantation endometrial leukocytes in women with recurrent miscarriage.
Human Reprod. 14(9):2386-2391. - Hauser, P.V. et al. (2010) Stem cells derived from human amniotic fluid contribute to acute kidney injury recovery.
Am J Pathol. 177: 2011-21. - Mallam, E. et al. (2010) Characterization of in vitro expanded bone marrow-derived mesenchymal stem cells from patients with multiple sclerosis.
Mult Scler. 16: 909-18. - Marrinucci, D. et al. (2010) Cytomorphology of circulating colorectal tumor cells:a small case series.
J Oncol. 2010: 861341. - Paul, G. et al. (2012) The adult human brain harbors multipotent perivascular mesenchymal stem cells.
PLoS One. 7: e35577. - De Schauwer, C. et al. (2012) In search for cross-reactivity to immunophenotype equine mesenchymal stromal cells by multicolor flow cytometry.
Cytometry A. 81 (4): 312-23. - Kazane, S.A. et al. (2012) Site-specific DNA-antibody conjugates for specific and sensitive immuno-PCR.
Proc Natl Acad Sci U S A. 109: 3731-6. - Spaas, J.H. et al. (2013) Culture and characterisation of equine peripheral blood mesenchymal stromal cells.
Vet J. 195 (1): 107-13.
Immunohistochemistry: Mouse Anti-Human MCP-1
. 4. Incubate the tissue section overnight at 4?C with Mouse Anti-Human MCP-1 at 10.0 μg/mL. Wash the slide twice for three minutes each. 5. Incubate the tissue section with the Envision HRP polymer kit (Dako) according to the manufacturer’s
Aspects of CNS Lupus: Mouse Models of Anti-NMDA Receptor Antibody Mediated Reactivity
transfer of pathology by injecting human and mouse brain-reactive antibodies into adult mouse as well as injecting the antibody into gestating mice and transfer of antibody from dam to fetus, (5) blocking NMDAR-mediated pathogenicity in vivo, (6) evacuation
Intraspinal Transplantation of Mouse and Human Neural Precursor Cells
the preparation and transplantation of human neural precursor cells (hNPCs) and mouse neural precursor cells (mNPCs) into the thoracic region of the mouse spinal cord. The techniques in this unit also describe how to prepare the mouse for surgery by performing
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