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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
CBAT6 thymocytes
- 亚型:
IgG2a
- 形态:
Purified IgG conjugated to StarBright UltraViolet 605 - liquid
- 克隆性:
Monoclonal Antibody
- 标记物:
StarBright UltraViolet 605
- 适应物种:
Mouse
- 保质期:
自发货之日起 12 个月
- 目录编号:
KT3
- 供应商:
伯乐生命医学产品(上海)有限公司
- 应用范围:
F
- 浓度:
For information on the concentration of our StarBright Dye conjugated reagents please visit our FAQ page.
- 抗体名:
CD3 antibody | KT3
- 规格:
100 Tests/0.5ml
NB. For optimal staining incubations should be performed at room temperature.
储存信息
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
应用
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ | Neat |
Flow Cytometry
Use 5ul of the suggested working dilution to label 106 cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
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文献和实验Source Reference
- Tomonari, K. & Lovering, E. (1988) T-cell receptor-specific monoclonal antibodies against a V beta 11-positive mouse T-cell clone.
Immunogenetics. 28 (6): 445-51.
References for CD3 antibody
- Tomonari, K. (1988) A rat antibody against a structure functionally related to the mouse T-cell receptor/T3 complex.
Immunogenetics. 28 (6): 455-8. - Lazarovits, A.I. et al. (1999) Mechanisms of induction of renal allograft tolerance in CD45RB-treated mice.
Kidney Int. 55: 1303-10. - Heitmann, S. et al. (1999) Immunohistological characterization of leukocytes in the lungs of healthy mice and after bacterial intratracheal infection.
Lab Anim. 33: 288-94. - Tarlton, J.F. et al. (2000) The role of up-regulated serine proteases and matrix metalloproteinases in the pathogenesis of a murine model of colitis.
Am J Pathol. 157: 1927-35. - Lacroix-Lamandé, S. et al. (2002) Role of gamma interferon in chemokine expression in the ileum of mice and in a murine intestinal epithelial cell line after Cryptosporidium parvum infection.
Infect Immun. 70 (4): 2090-9. - Kumar, L. et al. (2002) Differential role of SLP-76 domains in T cell development and function.
Proc Natl Acad Sci U S A. 99: 884-9. - Hare, K.J. et al. (2003) Modeling TCR signaling complex formation in positive selection.
J Immunol. 171: 2825-31. - Erlandsson, L. et al. (2004) Impaired B-1 and B-2 B cell development and atypical splenic B cell structures in IL-7 receptor-deficient mice.
Eur J Immunol. 34: 3595-603.
saline (PBS) (Cat. no. 10010-049) or equivalent Optional: ArC™ Amine Reactive Compensation Bead Kit (Cat. no. A10346) 实验步骤 1. Using the AbC™ anti- Rat/Hamster Bead Kit 1) Completely resuspend the AbC™ RH
分别利用 CD3 与 CD28 的功能学抗体,以及 CD3/CD28 Streptamer® 激活扩增 T 细胞的实验步骤与实验结果。 CD3/CD28抗体法 1、实验步骤: 1.1 抗体包被 用无菌 PBS 将 anti-mouse CD3 抗体(克隆号:145-2C11)稀释至 5μg/ml,稀释后的抗体加入到 24 孔板中,每孔 400μl,4°C 包被过夜。(注:板子在加入抗体之前,先用无菌 PBS 润洗 2-3 遍,避免干孔); 1.2 小鼠脾脏淋巴细胞分离(次日) 1. 将 70μm
RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
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