- 询价
- BIO-RAD
- MCA1266SBR670
- 2025年12月11日
- F
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Spleen and bone marrow cells from CE mice.
- 亚型：
IgG2a
- 形态：
Purified IgG conjugated to StarBright Red 670 - liquid
- 克隆性：
Monoclonal Antibody
- 标记物：
StarBright Red 670
- 适应物种：
Mouse
- 保质期：
自发货之日起 12 个月
- 目录编号：
PK136
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
F
- 浓度：
For information on the concentration of our StarBright Dye conjugated reagents please visit our FAQ page.
- 抗体名：
CD161 / NK1.1 antibody | PK136
- 规格：
100 Tests/0.5ml
Mouse anti Mouse CD161 / NK1.1 antibody, clone PK136 recognizes the mouse NK1.1 cell surface antigen, a cell surface glycoprotein encoded by members of the NKR-P1 gene family. The NK1.1 surface antigen is also known as CD161b/CD161c and Ly-55.
In the mouse the NKR-P1 family has three members, NKR-P1A, -B and -C, whilst in the human only one member has been identified. The human protein has received the designation CD161, and the mouse proteins have been referred to as CD161a, -b, -c etc.
Although previously thought to recognize only CD161c, recent data has shown that the PK136 antibody may also react with CD161b. CD161c expression itself is strain specific in mice, but recognition of CD161b by PK136 appears to be even more complex, as only some CD161b positive strains are labelled by the antibody. Engagement of CD161c has been reported to have activating function in NK cells, whilst engagement of CD161b is inhibitory.
Mouse anti Mouse NK1.1 Antigen antibody, clone PK136 is useful for the identification of NK cells in selected strains of mice (positive on C57BL, FVB/N and NZB, but negative on AKR and BALB/c) and is also expressed by rare subsets of T cells and monocytes. Mouse anti Mouse NK1.1 antibody, clone PK136 has also been used for in vivo depletion of NK cells (Wang et al. 2022) and in vitro activation of NK cells (Kung and Miller 1995).
In the mouse the NKR-P1 family has three members, NKR-P1A, -B and -C, whilst in the human only one member has been identified. The human protein has received the designation CD161, and the mouse proteins have been referred to as CD161a, -b, -c etc.
Although previously thought to recognize only CD161c, recent data has shown that the PK136 antibody may also react with CD161b. CD161c expression itself is strain specific in mice, but recognition of CD161b by PK136 appears to be even more complex, as only some CD161b positive strains are labelled by the antibody. Engagement of CD161c has been reported to have activating function in NK cells, whilst engagement of CD161b is inhibitory.
Mouse anti Mouse NK1.1 Antigen antibody, clone PK136 is useful for the identification of NK cells in selected strains of mice (positive on C57BL, FVB/N and NZB, but negative on AKR and BALB/c) and is also expressed by rare subsets of T cells and monocytes. Mouse anti Mouse NK1.1 antibody, clone PK136 has also been used for in vivo depletion of NK cells (Wang et al. 2022) and in vitro activation of NK cells (Kung and Miller 1995).
储存信息
This product is shipped at ambient temperature.
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ | Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 5μl of the suggested working dilution to label 106 cells in 100μl. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
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文献和实验该产品被引用文献
Source Reference
- Koo, G.C. & Peppard, J.R. (1984) Establishment of monoclonal anti-Nk-1.1 antibody.
Hybridoma. 3 (3): 301-3.
References for CD161 / NK1.1 antibody
- Koo, G.C. et al. (1986) The NK-1.1(-) mouse: a model to study differentiation of murine NK cells.
J Immunol. 137 (12): 3742-7. - Kung, S.K. & Miller RG (1995) The NK1.1 antigen in NK-mediated F1 antiparent killing in vitro.
J Immunol. 154 (4): 1624-33. - Wang, M. et al. (1998) Natural killer cell depletion fails to influence initial CD4 T cell commitment in vivo in exogenous antigen-stimulated cytokine and antibody responses.
J Immunol. 160 (3): 1098-105. - Halin, C. et al. (2002) Enhancement of the antitumor activity of interleukin-12 by targeted delivery to neovasculature.
Nat Biotechnol. 20 (3): 264-9. - Carnemolla, B. et al. (2002) Enhancement of the antitumor properties of interleukin-2 by its targeted delivery to the tumor blood vessel extracellular matrix.
Blood. 99: 1659-65. - Svensson, L. et al. (2003) gammadelta T cells contribute to the systemic immunoglobulin E response and local B-cell reactivity in allergic eosinophilic airway inflammation.
Immunology. 108 (1): 98-108. - Ebbinghaus, C. et al. (2005) Engineered vascular-targeting antibody-interferon-gamma fusion protein for cancer therapy.
Int J Cancer. 116 (2): 304-13. - Joseph-Pietras, D. et al. (2006) Anti-tumoural activity of peripheral blood mononuclear cells against melanoma cells: discrepant in-vitro and in-vivo effects.
Melanoma Res. 16: 325-33.
Further Reading
- Arase, N. et al. (1997) Association with FcRgamma is essential for activation signal through NKR-P1 (CD161) in natural killer (NK) cells and NK1.1+ T cells.
J Exp Med. 186 (12): 1957-63.
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Mouse anti Mouse CD161 / NK1.1:StarBright Red 670
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