- 询价
- BIO-RAD
- MCA2678SBUV575
- 2025年12月11日
- F
- Bovine
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Partially purified polypeptides isolated from bovine leucocyte cell surface membrane.
- 亚型：
IgG1
- 形态：
Purified IgG conjugated to StarBright UltraViolet 575 - liquid
- 克隆性：
Monoclonal Antibody
- 标记物：
StarBright UltraViolet 575
- 适应物种：
Bovine
- 保质期：
自发货之日起 12 个月
- 目录编号：
CC-G33
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
F
- 浓度：
For information on the concentration of our StarBright Dye conjugated reagents please visit our FAQ page.
- 抗体名：
CD14 antibody | CC-G33
- 规格：
100 Tests/0.5ml
Mouse anti Bovine CD14, clone CC-G33 recognizes bovine CD14.
CD14 is a GPI-anchored membrane glycoprotein and monocyte/macrophage differentiation antigen, belonging to the lipopolysaccharide receptor family, also expressed weakly on microglia and Langerhans cells. CD14 acts as a receptor for the potent bacterial endotoxin, lipopolysaccharide (LPS), facilitated by LPS-binding protein (LBP). The binding of LPS to CD14 results in cell activation and the release of cytokines and the inflammatory response, and has been shown to upregulate the cell surface expression of adhesion molecules.
Mouse anti Bovine CD14 clone CC-G33 cross-reacts with human CD14 expressed on transfected COS-7 cells (Berthon & Hopkins 1996), ovine CD14 (Sopp et al. 1996) and Water buffalo (Bubalus bubalis) CD14, (Mirielli et al. 2013).
CD14 is a GPI-anchored membrane glycoprotein and monocyte/macrophage differentiation antigen, belonging to the lipopolysaccharide receptor family, also expressed weakly on microglia and Langerhans cells. CD14 acts as a receptor for the potent bacterial endotoxin, lipopolysaccharide (LPS), facilitated by LPS-binding protein (LBP). The binding of LPS to CD14 results in cell activation and the release of cytokines and the inflammatory response, and has been shown to upregulate the cell surface expression of adhesion molecules.
Mouse anti Bovine CD14 clone CC-G33 cross-reacts with human CD14 expressed on transfected COS-7 cells (Berthon & Hopkins 1996), ovine CD14 (Sopp et al. 1996) and Water buffalo (Bubalus bubalis) CD14, (Mirielli et al. 2013).
储存信息
This product is shipped at ambient temperature.
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ | Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 5μl of the suggested working dilution to label 106 cells in 100μl. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
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文献和实验该产品被引用文献
Source Reference
- Sopp, P. et al. (1996) Identification of bovine CD14.
Vet Immunol Immunopathol. 52 (4): 323-8.
References for CD14 antibody
- Berthon, P. & Hopkins, J. (1996) Ruminant cluster CD14.
Vet Immunol Immunopathol. 52 (4): 245-8. - Haas, K.M. and Estes, D.M. (2001) The identification and characterization of a ligand for bovine CD5.
J Immunol. 166: 3158-66. - Altreuther, G. et al. (2001) Morphologic and functional changes in bovine monocytes infected in vitro with the bovine leukaemia virus.
Scand J Immunol. 54: 459-69. - Fikri Y et al. (2002) Costimulatory molecule requirement for bovine WC1+gammadelta T cells' proliferative response to bacterial superantigens.
Scand J Immunol. 55 (4): 373-81. - Glew, E.J. et al. (2003) Differential effects of bovine viral diarrhoea virus on monocytes and dendritic cells.
J Gen Virol. 84: 1771-80. - Harris, J. et al. (2003) Expression of caveolin by bovine lymphocytes and antigen-presenting cells.
Immunology. 105: 190-5. - Villarreal-Ramos, B. et al. (2003) Investigation of the role of CD8+ T cells in bovine tuberculosis in vivo.
Infect Immun.71: 4297-303. - Gliddon, D.R. et al. (2004) DEC-205 expression on migrating dendritic cells in afferent lymph.
Immunology. 11: 262-72.
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Mouse anti Bovine CD14:StarBright UltraViolet 575
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