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- 详细信息
- 文献和实验
- 技术资料
- 库存:
1000
- 供应商:
广州艾迪基因科技有限责任公司
- 肿瘤类型:
详见说明
- 细胞类型:
详见说明
- 品系:
详见说明
- 组织来源:
详见说明
- 相关疾病:
详见说明
- 物种来源:
人
- 免疫类型:
详见说明
- 细胞形态:
详见说明
- 是否是肿瘤细胞:
详见说明
- 器官来源:
详见说明
- 运输方式:
液氮保存
- 生长状态:
详见说明
- 规格:
1x10^6 cells
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文献和实验acceptor, such the green fluorescent protein (GFP), and the two are then coexpressed in the same cells. If the two proteins interact, their close proximity allows nonradiative energy transfer (BRET) between the luciferase and the GFP. BRET does not occur
Plant DNA methylation analysis by bisulfite genomic sequencing
) at temperature appropriate for used restriction enzyme; Add 0.5ml Tris-buffer-saturated phenol-chroroform, vortex; Centrifuge with Eppendorf 5414 or equivalent at 10000 x g for 2 minutes; Transfer the upper aqueous phase to clean 1.5ml
I and Nhe I and ligated with 3 g of Xma I- and Nhe I-digested inserts to generate the pComb3X-6ZF library vector. Both 3ZF and 6ZF library inserts were digested with SfI I and subcloned into the retroviral vector pMX-IRES-GFP, containing the VP64 activation
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