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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
CD8a
- 形态:
Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
- 保存条件:
避光,4-8℃
- 克隆性:
REAL137
- 标记物:
VioBlue
- 适应物种:
mouse
- 保质期:
24个月
- 供应商:
Miltenyi biotec
- 宿主:
cell line
- 应用范围:
Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry
- 浓度:
1:50
- 抗体英文名:
CD8a Antibody, anti-mouse, REAlease®
- 抗体名:
CD8a Antibody, anti-mouse, REAlease®
- 规格:
30 µg in 200 µL
Identification and enumeration of CD8a+ cells by flow cytometryClone REAL137 is an antibody fragment derived from the full CD8a antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity. | Clone REAL137 is specific for the mouse CD8a antigen, also known as LY-2 or T8, which is expressed on cytotoxic T cells and at lower levels on subpopulations of dendritic cells and TCRγ/δ+ cells. CD8a is further detected on most thymocytes (CD4+CD8a+ and CD4–CD8+ thymocytes). Cytotoxic T cells and thymocytes express CD8a as heterodimer with CD8b, whereas dendritic cells and TCRγ/δ+ cells express a CD8a/CD8a homodimer. It mediates efficient cell-cell interactions within the immune system. | The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.
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文献和实验该产品被引用文献
Nakauchi, H. et al. (1985) Molecular cloning of Lyt-2, a membrane glycoprotein marking a subset of mouse T lymphocytes: molecular homology to its human counterpart, Leu-2/T8, and to immunoglobulin variable regions. Proc. Natl. Acad. Sci. U.S.A. 82 (15): 5126–5130. | Liaw, C. W. et al. (1986) Structure, sequence, and polymorphism of the Lyt-2 T cell differentiation antigen gene. J Immunol 137 (3): 1037–1043. | Nakauchi, H. et al. (1987) Isolation and characterization of the gene for the murine T cell differentiation antigen and immunoglobulin-related molecule, Lyt-2. Nucleic Acids Res. 15 (10): 4337–4347.
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CD8a Antibody, anti-mouse, VioBlue®, REAlease®, 30 µg in 200 µL
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