Sca-1 Antibody, anti-mouse, FI

Identification and enumeration of Sca-1+ cells by flow cytometryClone REAL175 is an antibody fragment derived from the full Sca-1 antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity. | Clone REAL175 recognizes the mouse stem cell antigen-1 (Sca-1) antigen, an 18 kDa glycosylphosphatidylinositol (GPI) linked protein which is also known as lymphocyte antigen 6A and 6E (Ly-6A/E). Clone REAL175 specifically recognizes both Ly-6E.1 and Ly-6A.2, which are gene products of the two Ly-6A/E alleles expressed in different mouse strains (Ly-6E.1: e.g. BALB/c, C3H, NZB; Ly-6A.2: e.g. C57BL/6, SJL, 129, AKR). Sca-1 is expressed on hematopoietic stem cells and progenitor cells in mouse bone marrow and is one of the defining markers for so-called KSL (c-kit+ Sca-1+ Lin–) cells. In combination with CD105 (Endoglin) expression, Sca-1 has been used to define long-term repopulating hematopoietic stem cells (LTR-HSCs) in mouse bone marrow. In addition, Sca-1+ bone marrow cells also contain the mesenchymal stem cell fraction. Sca-1+ cells have been shown to give rise to hepatocytes in vivo and neural cells in vitro. Furthermore, Sca-1 is expressed on stem cells in a variety of nonhematopoietic tissues, such as adult liver, heart, and prostate. | The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.