Ly-49A Antibody, anti-mouse, A

Identification and enumeration of Ly-49A+ cells by flow cytometry.Clone REAL436 is an antibody fragment derived from the full Ly-49A antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity. | Clone REAL436 recognizes th mouse Ly49A antigen, an inhibitory cell surface receptor belonging to the Ly49 receptor family. Ly49A is a 44 kDa type-II transmembrane, disulfide-linked homodimer with C-type lectin extracellular domains. Expression of Ly49A is found on subset of NK and NK-T cells. Ly49 family members are both activating and inhibitory receptors and are mainly receptors for MHC class I molecules. Ly49A interacts strongly with MHC class Ia molecules such as H-2Dd, H-2Dk and weakly with H-2Db. An inhibitory signal is delivered by Ly49A engagement via the phosphorylation of immunoreceptor tyrosine based inhibition motif (ITIM) and recruitment of phosphatases SHP-1 and SHP-2. Recognition of cognate ligand bearing cells leads to inhibition of cytotoxic killing of cells by NK cells. | The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.