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CD97 Antibody, anti-human, APC

, REAfinity™, 30 tests in 60 µL
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  • 询价
  • Miltenyi Biotec已认证
  • 德国
  • 130-124-980
  • 2025年12月09日
  • Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry
  • human cell line
  • human
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      CD97

    • 亚型

      recombinant human IgG1

    • 形态

      Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.

    • 保存条件

      避光,4-8℃

    • 克隆性

      REA1242

    • 标记物

      APC

    • 适应物种

      human

    • 保质期

      24个月

    • 供应商

      Miltenyi biotec

    • 宿主

      human cell line

    • 应用范围

      Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry

    • 浓度

      1:50

    • 抗体英文名

      CD97 Antibody, anti-human, REAfinity™

    • 抗体名

      CD97 Antibody, anti-human, REAfinity™

    • 规格

      30 tests in 60 µL

    Identification and enumeration of CD97+ cells by flow cytometryClone REA1242 reacts with the human CD97 antigen, a member of the epidermal growth factor (EGF)-TM7 family of cell surface receptors. CD97 is expressed by various cell types of the hematopoietic system and also on smooth muscle cells. Highest constitutive expression is found on granulocytes and monocytes. Lymphocytes express CD97 at lower level (T and NK cells expressing higher levels than B cells) but on T cells CD97 is upregulated upon activation via CD3 and CD28 or PHA. CD97 is a ligand for CD55 (decay-accelerating factor, DAF) and has been shown to mediate cell adhesion and co-stimulation of T cell proliferation. | Additional information: Clone REA1242 displays negligible binding to Fc receptors.

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    图标文献和实验
    该产品被引用文献
    Hamann, J. et al. (1995) Expression cloning and chromosomal mapping of the leukocyte activation antigen CD97, a new seven-span transmembrane molecule of the secretion receptor superfamily with an unusual extracellular domain. J Immunol 155 (4): 1942–1950.
    相关实验
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      30s,接着40个循环的94℃ 15 s,65℃ 20 s,68℃ 5 min],其中使用了1单位的ELONGASE酶混合物[PCR 50ml终体积,其中60 mM Tris-SO4(pH9.1),18 mM (NH4)2SO4,2 mM MgSO4,dNTP每个200 µM,引物每个400 nM ],其中分别是AAP和引物TSC-2-2826(A带)或引物TSC-2-1732(B带)一起使用。每个PCR产物(带1和带3)取10µl 在0.8%琼脂糖上,1倍TAE凝胶电泳和SYBRò绿染色(分子

    • AbC Anti-Mouse Bead Kit

      实验步骤   1.  Using the AbC Anti-Mouse Bead Kit 1)        Completely resuspend the AbC capture beads (Component A) and negative beads (Component B) by gently vortexing for 30 seconds before use. 2)        Label

    • AbC Anti-Rat/Hamster Bead Kit

      1 μL   5)      Label another sample tube for each fluorochrome-conjugated antibody you are using, and add 1 drop of AbC capture beads (Component A in the AbC Anti-Mouse Bead Kit) to each tube. 6)     

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    文献支持
    CD97 Antibody, anti-human, APC, REAfinity™, 30 tests in 60 µL
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