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CD90.1 Antibody, anti-mouse/ra

t, PE-Vio® 770, REAfinity™, 30 µg in 200 µL
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  • 询价
  • Miltenyi Biotec已认证
  • 德国
  • 130-112-875
  • 2025年12月09日
  • Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry
  • human cell line
  • mouse, rat
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德国美天旎生物技术有限公司
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原：

      CD90.1

    • 亚型：

      recombinant human IgG1

    • 形态：

      Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.

    • 保存条件：

      避光，4-8℃

    • 克隆性：

      REA838

    • 标记物：

      PE-Vio 770

    • 适应物种：

      mouse, rat

    • 保质期：

      24个月

    • 供应商：

      Miltenyi biotec

    • 宿主：

      human cell line

    • 应用范围：

      Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry

    • 浓度：

      1:50

    • 抗体英文名：

      CD90.1 Antibody, anti-mouse/rat, REAfinity™

    • 抗体名：

      CD90.1 Antibody, anti-mouse/rat, REAfinity™

    • 规格：

      30 µg in 200 µL

    Identification and enumeration of CD90.1+ cells by flow cytometryClone REA838 recognizes the rat CD90 (Thy-1) and mouse CD90.1 (Thy1.1) antigen, which belong to a GPI-anchored conserved membrane glycoprotein. In the mouse strains AKR/J, PL, and FVB/N, CD90.1 is a pan T cell marker and can be found on thymocytes, hematopoietic stem cells in the bone marrow, intra-epithelial cells (dendritic epidermal cells) in skin, and on neurons such as retinal ganglion cells. In rat, the CD90 antigen is expressed on thymocytes, recent thymic emigrants, hematopoietic stem cells, on neurons such as retinal ganglion cells, and on other cell types. The antibody does not cross-react with CD90.2 (Thy1.2). | Additional information: Clone REA838 displays negligible binding to Fc receptors.

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    图标文献和实验
    该产品被引用文献
    Seki, T. et al. (1985) Thy-1: a hydrophobic transmembrane segment at the carboxyl terminus. Fed. Proc. 44 (13): 2865–2869. | Seki, T. et al. (1985) Structural organization of the rat thy-1 gene. Nature 313 (6002): 485–487. | Ishizu, A. et al. (1995) Thy-1 induced on rat endothelium regulates vascular permeability at sites of inflammation. Int. Immunol. 7 (12): 1939–1947.
    相关实验

    • Coating of Platelets with Antibody in vitro

      or an Ab directed to platelet Ag (you may use Grener F-bottom microtitration plate for this step). If the Ab used is rat IgG1 (k) against mouse CD41 (MWReg30, Becton Dickinson, Cat.: 09911D) then the advised concentration is 0.6µg/ml (0.15µg per 200µl

    • Immunofluorescent Staining of Mouse and Rat Leukocytes

      (or 50 µl wash buffer for negative controls). Mix by gently vortexing or tapping. Incubate at 4°C for 20-40 min in the dark. Wash 2X with 200 µl wash buffer (or 3X if a biotin-conjugated primary antibody is used). After each centrifugation, 350 x g

    • 原创：western protocol

      1.5M Tris(PH 8.8) 100ml 18.171g (at4°C) 10% SDS 100 ml 10g (at room temperature) 10% ammonium persulfate 1ml 0.1g(replace every week store at at4°C) TEMED 8 room temprature Antibodies Primary antibody: 5A11(anti-mouse IgG) 500x-1000x Secondary

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    文献支持
    CD90.1 Antibody, anti-mouse/rat, PE-Vio® 770, REAfinity™, 30 µg in 200 µL
    询价