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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
CD68
- 亚型:
recombinant human IgG1
- 形态:
Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
- 保存条件:
避光,4-8℃
- 克隆性:
REA835
- 标记物:
PerCP-Vio 700
- 适应物种:
mouse
- 保质期:
24个月
- 供应商:
Miltenyi biotec
- 宿主:
human cell line
- 应用范围:
Intracellular flow cytometry, MICS (MACSima Imaging Cyclic Staining), 3D-Immunofluorescence, Immunofluorescence, Immunohistochemistry
- 浓度:
1:50
- 抗体英文名:
CD68 Antibody, anti-mouse, REAfinity™
- 抗体名:
CD68 Antibody, anti-mouse, REAfinity™
- 规格:
150 µg in 1 mL
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文献和实验antibody: Anti-mouse IgG-Alkaline Phospphatase Buffers Lysis buffer: 100ml 50 mM Tris-Cl, pH 8.0 0.6057g 150m M NaCl 0.8766g 0.02%NaN3 0.02g 100ug/mlPMSF 0.01g 10ug/ml Aprotinin 0.001g 1% Triton X100 1g 2x SDS-loading buffer: 100ml 100 mM Tris
with the better the chances of a successful pulldown. A total of 4.0×106 293 HEK cells (~70% confluent) are plated and 24 hours later transfected with 15µg of a RNA Polymerase II expressing flag-tagged construct plasmid. We generally use Lipofectamine 2000™
The production of monoclonal antibody by hybridoma
powder supplied by SIGMA at -700C until needed. Reconstitute 1 vial / 500 ml of media and add entire contents to 500 ml media (eg. 2 vials/ litre). Myeloma Media is CM which has 10% FBS (or HS) and 8-Aza (1 X) stored
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