- 询价
- Miltenyi Biotec
- 德国
- 130-125-850
- 2025年12月09日
- Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry, Immunocytochemistry
- human cell line
- human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
CD66c
- 亚型：
recombinant human IgG1
- 形态：
Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
- 保存条件：
避光，4-8℃
- 克隆性：
REA414
- 标记物：
APC
- 适应物种：
human
- 保质期：
24个月
- 供应商：
Miltenyi biotec
- 宿主：
human cell line
- 应用范围：
Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry, Immunocytochemistry
- 浓度：
1:50
- 抗体英文名：
CD66c Antibody, anti-human, REAfinity™
- 抗体名：
CD66c Antibody, anti-human, REAfinity™
- 规格：
100 tests in 200 µL
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文献和实验30s，接着40个循环的94℃ 15 s，65℃ 20 s，68℃ 5 min]，其中使用了1单位的ELONGASE酶混合物[PCR 50ml终体积，其中60 mM Tris－SO4（pH9.1），18 mM (NH4)2SO4，2 mM MgSO4，dNTP每个200 µM，引物每个400 nM ]，其中分别是AAP和引物TSC－2－2826（A带）或引物TSC－2－1732（B带）一起使用。每个PCR产物（带1和带3）取10µl 在0.8％琼脂糖上，1倍TAE凝胶电泳和SYBRò绿染色（分子
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
of 0.3% SDS). Mix by gentle pipetting. Shake gently for 1 h at 37°C. 15. Add 50 µL of 20% Triton X-100 (to a final concentration of 1.8%). Shake gently for 1 h at 37°C. 16. Add 1200 units of restriction enzyme (i.e., 12 µL
IMMUNOSCREENING OF WITH ANTIBODY PROBES
Solution 12.5 mg/ml in 100% dimethylformamide. Store in a foil wrapped bottle at 4°C. (85% dimethylformamide also works fine to dissolve the BCIP).(Promega sells the NBT & BCIP solutions both @ 50 mg/ml & suggests using 66µl NBT
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