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CD59 Antibody, anti-rat, APC,

REAfinity™, 9 µg in 300 µL
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  • 询价
  • Miltenyi Biotec已认证
  • 德国
  • 130-106-861
  • 2025年12月09日
  • Flow cytometry
  • human cell line
  • rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      CD59

    • 亚型

      recombinant human IgG1

    • 形态

      Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.

    • 保存条件

      避光,4-8℃

    • 克隆性

      REA452

    • 标记物

      APC

    • 适应物种

      rat

    • 保质期

      24个月

    • 供应商

      Miltenyi biotec

    • 宿主

      human cell line

    • 应用范围

      Flow cytometry

    • 浓度

      1:10

    • 抗体英文名

      CD59 Antibody, anti-rat, REAfinity™

    • 抗体名

      CD59 Antibody, anti-rat, REAfinity™

    • 规格

      9 µg in 300 µL

    Identification and enumeration of CD59+ cells by flow cytometryClone REA452 recognizes the rat CD59 antigen, a 21 kDa glycosylphosphatidylinositol (GPI) linked cell-surface glycoprotein which is also known as MAC-inhibitory protein (MAC-IP), membrane attack complex inhibition factor (MACIF), or protectin. CD59 is widely distributed, being present on all epithelia, endothelia, and on circulating cells, including erythrocytes, monocytes, and some lymphocytes. It inhibits the lytic activity of complement by binding to C8 and C9, thereby blocking the incorporation of mutiple C9 molecules into the membrane attack complex thus preventing functional pore formation. | Additional information: Clone REA452 displays negligible binding to Fc receptors.

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    图标文献和实验
    该产品被引用文献
    Rushmere, N. K. et al. (1994) Molecular cloning of the rat analogue of human CD59: structural comparison with human CD59 and identification of a putative active site. Biochem. J. 304 (Pt 2): 595–601. | Rushmere, N. K. et al. (1997) Expression of rat CD59: functional analysis confirms lack of species selectivity and reveals that glycosylation is not required for function. Immunology 90 (4): 640–646. | Mizuno, T. et al. (2011) Specific collaboration between rat membrane complement regulators Crry and CD59 protects peritoneum from damage by autologous complement activation. Nephrol Dial Transplant 26 (6): 1821–1830.
    相关实验
    • AbC Anti-Rat/Hamster Bead Kit

      1 μL   5)      Label another sample tube for each fluorochrome-conjugated antibody you are using, and add 1 drop of AbC capture beads (Component A in the AbC Anti-Mouse Bead Kit) to each tube. 6)     

    • Immunofluorescent Staining of Mouse and Rat Leukocytes

      secondary reagent (e.g., fluorochrome-conjugated avidin, streptavidin, anti-Ig allotype, anti-Ig isotype, polyclonal anti-Ig). For example, dilute antibody to ~1 µg per 100 µl in wash buffer and add this to each well containing the loosened cell pellet

    • Anti-GFP/BrdU In Situ Immunostaining

      Dilute 1 µL fresh streptavidin-Alexa Flour 555 in 1 mL PBS (1:1,000).  Spin the dilute antibody at high speed for 30'  to remove particulates.  Add supernatant to specimen and incubate 30' at room temp.  Remove antibody and add 1 mL PBS.  O.K

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    文献支持
    CD59 Antibody, anti-rat, APC, REAfinity™, 9 µg in 300 µL
    询价