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CD49d Antibody, anti-human, PE

-Vio® 670, REAfinity™, 100 tests in 200 µL
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  • 询价
  • Miltenyi Biotec已认证
  • 德国
  • 130-133-747
  • 2025年12月09日
  • Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry
  • human cell line
  • human, non-human primate, other
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  • 企业认证

    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      CD49d

    • 亚型

      recombinant human IgG1

    • 形态

      Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.

    • 保存条件

      避光,4-8℃

    • 克隆性

      REA545

    • 标记物

      PE-Vio 670

    • 适应物种

      human, non-human primate, other

    • 保质期

      24个月

    • 供应商

      Miltenyi Biotec

    • 宿主

      human cell line

    • 应用范围

      Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry

    • 浓度

      1:50

    • 抗体英文名

      CD49d Antibody, anti-human, REAfinity™

    • 抗体名

      CD49d Antibody, anti-human, REAfinity™

    • 规格

      100 tests in 200 µL

    Identification and enumeration of CD49d+ cells by flow cytometryClone REA545 recognizes the human CD49d antigen, which belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. CD49d, also known as the α4 integrin chain or VLA-4α chain, is a cell adhesion protein that is directly involved in mononuclear leukocyte trafficking. Dimerized with β7 integrin, the resulting α4β7 integrin binds VCAM-1 (CD106), MAdCAM-1, and fibronectin to facilitate the rolling of leukocytes along vascular epithelium. In T cells, on ligation of MAdCAM-1, α4β7 integrin also provides costimulation of T cell receptor/CD3–mediated signaling. | CD49d is expressed on most mononuclear leukocytes, both in circulation or in lymphoid or other tissues, as well as on eosinophils and basophils. | Additional information: Clone REA545 displays negligible binding to Fc receptors.

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    图标文献和实验
    该产品被引用文献
    Bulian, P. et al. (2014) CD49d is the strongest flow cytometry–based predictor of overall survival in chronic lymphocytic leukemia. J. Clin. Oncol. 32: 897–904. | Yoshino, N. et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies. Exp. Anim. 49 (2): 97–110.
    相关实验
    • Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum

      with deionized water, and mix Mouse osteocalcin standard, one vial, 200 ng, lyophilized: reconstitute standard with exactly 2.0 ml of sample buffer (yields a 100 ng/ml solution); use

    • AbC Anti-Rat/Hamster Bead Kit

      1 μL   5)      Label another sample tube for each fluorochrome-conjugated antibody you are using, and add 1 drop of AbC capture beads (Component A in the AbC Anti-Mouse Bead Kit) to each tube. 6)     

    • ELISA Procedure for Measuring Serum Antibody Titer

      Dilution ScheduleDilution ofAntiserum Volume of 1% BSA/PBS-T (µL) Volume of PreviousDilution (µL) 1:100 1000 1000 of 1:50 1:200 1000 1000 of 1:100 1:400 1000 1000 of 1:200 1:800 1000 1000 of 1:400 ... ... ... 1:102,400 1000 1000 of 1:51,200 11. Vortex

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    文献支持
    CD49d Antibody, anti-human, PE-Vio® 670, REAfinity™, 100 tests in 200 µL
    询价