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CD49b Antibody, anti-mouse/rat

, APC, REAfinity™, 30 µg in 200 µL
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  • 询价
  • Miltenyi Biotec已认证
  • 德国
  • 130-116-433
  • 2025年12月09日
  • Flow cytometry
  • human cell line
  • mouse, rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      CD49b

    • 亚型

      recombinant human IgG1

    • 形态

      Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.

    • 保存条件

      避光,4-8℃

    • 克隆性

      REA981

    • 标记物

      APC

    • 适应物种

      mouse, rat

    • 保质期

      24个月

    • 供应商

      Miltenyi Biotec

    • 宿主

      human cell line

    • 应用范围

      Flow cytometry

    • 浓度

      1:50

    • 抗体英文名

      CD49b Antibody, anti-mouse/rat, REAfinity™

    • 抗体名

      CD49b Antibody, anti-mouse/rat, REAfinity™

    • 规格

      30 µg in 200 µL

    Identification and enumeration of CD49b+ cells by flow cytometryClone REA981 recognizes the mouse and rat CD49b antigen, a transmembrane glycoprotein also known as integrin alpha-2 (Itga2). CD49 is found on activated lymphocytes, epithelial cells, and platelets. In association with CD29 (integrin β-1), a VLA-2 complex is formed, which functions as a receptor for collagen and collagen C-propeptides, laminin, and E-cadherin. | Additional information: Clone REA981 displays negligible binding to Fc receptors.

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    图标文献和实验
    该产品被引用文献
    Hemler, M. E. et al. (1990) LA proteins in the integrin family: structures, functions, and their role on leukocytes. Annu. Rev. Immunol. 8: 365–400. | Wu, J. E. et al. (1994) Complex patterns of expression suggest extensive roles for the alpha 2 beta 1 integrin in murine development. Dev. Dyn. 199 (4): 292–314.
    相关实验
    • APC和TSC-2 cDNA的长5'RACE

      30s,接着40个循环的94℃ 15 s,65℃ 20 s,68℃ 5 min],其中使用了1单位的ELONGASE酶混合物[PCR 50ml终体积,其中60 mM Tris-SO4(pH9.1),18 mM (NH4)2SO4,2 mM MgSO4,dNTP每个200 µM,引物每个400 nM ],其中分别是AAP和引物TSC-2-2826(A带)或引物TSC-2-1732(B带)一起使用。每个PCR产物(带1和带3)取10µl 在0.8%琼脂糖上,1倍TAE凝胶电泳和SYBRò绿染色(分子

    • Coating of Platelets with Antibody in vitro

      or an Ab directed to platelet Ag (you may use Grener F-bottom microtitration plate for this step). If the Ab used is rat IgG1 (k) against mouse CD41 (MWReg30, Becton Dickinson, Cat.: 09911D) then the advised concentration is 0.6µg/ml (0.15µg per 200µl

    • Immunofluorescent Staining of Mouse and Rat Leukocytes

      (or 50 µl wash buffer for negative controls). Mix by gently vortexing or tapping. Incubate at 4°C for 20-40 min in the dark. Wash 2X with 200 µl wash buffer (or 3X if a biotin-conjugated primary antibody is used). After each centrifugation, 350 x g

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    文献支持
    CD49b Antibody, anti-mouse/rat, APC, REAfinity™, 30 µg in 200 µL
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