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CD45RO Antibody, anti-human, V

io® Bright B515, REAfinity™, 100 tests in 200 µL
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  • 询价
  • Miltenyi Biotec已认证
  • 德国
  • 130-137-195
  • 2025年12月09日
  • Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry, Mass cytometry
  • human cell line
  • human, non-human primate
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  • 企业认证

    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      CD45RO

    • 亚型

      recombinant human IgG1

    • 形态

      Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.

    • 保存条件

      避光,4-8℃

    • 克隆性

      REA611

    • 标记物

      Vio Bright B515

    • 适应物种

      human, non-human primate

    • 保质期

      24个月

    • 供应商

      Miltenyi Biotec

    • 宿主

      human cell line

    • 应用范围

      Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry, Mass cytometry

    • 浓度

      1:50

    • 抗体英文名

      CD45RO Antibody, anti-human, REAfinity™

    • 抗体名

      CD45RO Antibody, anti-human, REAfinity™

    • 规格

      100 tests in 200 µL

    Identification and enumeration of CD45RO+ cells by flow cytometryClone REA611 recognizes the human CD45RO antigen, a 180 kDa single-chain membrane glycoprotein, which is an isoform of CD45. CD45RO is expressed by most thymocytes, activated and memory T cells, granulocytes, B cell subsets, and monocytes. CD45RO binds to galectin-1 and CD22. CD45RO antibodies are commonly used in combination with antibodies against CD45RA to discriminate memory T cells from naive T cells. | Additional information: Clone REA611 displays negligible binding to Fc receptors.

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    图标文献和实验
    该产品被引用文献
    Charbonneau, H. et al. (1988) The leukocyte common antigen (CD45): a putative receptor-linked protein tyrosine phosphatase. Proc. Natl. Acad. Sci. U.S.A. 85 (19): 7182–7186. | Trowbridge, I. S. et al. (1994) CD45: an emerging role as a protein tyrosine phosphatase required for lymphocyte activation and development. Annu. Rev. Immunol. 12: 85–116.
    相关实验
    • Isolation of Untouched Human B Cells by Depletion of Non-B Cells

      in the same volume of Isolation Buffer as the initial volume of Dynabeads ®(step 2). 2.        Isolation Procedure 1)        Transfer 500 μl (5 × 107 ) PBMC in Isolation Buffer to a tube. 2)        Add 100 μl of Antibody Mix. 3)        Mix

    • AbC Anti-Rat/Hamster Bead Kit

      1 μL   5)      Label another sample tube for each fluorochrome-conjugated antibody you are using, and add 1 drop of AbC capture beads (Component A in the AbC Anti-Mouse Bead Kit) to each tube. 6)     

    • AbC Anti-Mouse Bead Kit

      实验步骤   1.  Using the AbC Anti-Mouse Bead Kit 1)        Completely resuspend the AbC capture beads (Component A) and negative beads (Component B) by gently vortexing for 30 seconds before use. 2)        Label

    图标技术资料

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    文献支持
    CD45RO Antibody, anti-human, Vio® Bright B515, REAfinity™, 100 tests in 200 µL
    询价