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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
CD4 (Domain 2)
- 亚型:
recombinant human IgG1
- 形态:
Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
- 保存条件:
避光,4-8℃
- 克隆性:
REA489
- 标记物:
APC-Vio 770
- 适应物种:
rat
- 保质期:
24个月
- 供应商:
Miltenyi Biotec
- 宿主:
human cell line
- 应用范围:
Flow cytometry
- 浓度:
1:10
- 抗体英文名:
CD4 (Domain 2) Antibody, anti-rat, REAfinity™
- 抗体名:
CD4 (Domain 2) Antibody, anti-rat, REAfinity™
- 规格:
9 µg in 300 µL
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文献和实验. 2. Combining AbC™ anti-Rat/Hamster and ArC™ Amine Reactive Compensation Bead Kits The ArC™ Amine Reactive Compensation Bead Kit (Cat. no. A10346) is designed to facilitate compensation when using any of the LIVE/DEAD® fixable dead cell stains
Th17 Polarization of Mouse CD4 Cells
;g/ml). Incubate at 37 °C for 2 hours or 4 °C over night. A septically decant antibody solution from the plate. Wash plate 3 times with sterile PBS. Discard liquid. 2) Plate CD4 cells at 1x106/ml. Culture cells for 3 days in presence
in 100µl of elution buffer at 65°C for 5 minutes;7.The eluted complexes can then be electrophoresed in denaturing PAGE and subjected to western blot analysis with an anti-Flag antibody for detection of the particular flag-tagged protein of choice (Figure
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