- 询价
- Miltenyi Biotec
- 德国
- 130-112-043
- 2025年12月09日
- Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry, Mass cytometry
- human cell line
- human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
CD304 (BDCA-4)
- 亚型：
recombinant human IgG1
- 形态：
Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
- 保存条件：
避光，4-8℃
- 克隆性：
REA774
- 标记物：
Biotin
- 适应物种：
human
- 保质期：
24个月
- 供应商：
Miltenyi Biotec
- 宿主：
human cell line
- 应用范围：
Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry, Mass cytometry
- 浓度：
1:50
- 抗体英文名：
CD304 (BDCA-4) Antibody, anti-human, REAfinity™
- 抗体名：
CD304 (BDCA-4) Antibody, anti-human, REAfinity™
- 规格：
30 tests in 60 µL
Identification and enumeration of CD304 (BDCA-4)+ cells by flow cytometryClone REA774 recognizes the human CD304 (BDCA-4) antigen. Like CD303 (BDCA-2), the CD304 (BDCA-4) antigen is specifically expressed on human plasmacytoid dendritic cells in blood and bone marrow. Unlike CD303 (BDCA-2), expression of CD304 (BDCA-4) is up-regulated on plasmacytoid dendritic cells and induced on myeloid cells upon culturing. In inflamed tonsils, CD304 expression, apart from plasmacytoid dendritic cells, is also detected primarily on follicular B helper memory T cells. The CD304 (BDCA-4) is identical to neuropilin-1 (NP-1), a neuronal receptor, which is known to be expressed on numerous nonhematopoietic cell types, including neurons, endothelial, and tumor cells. CD1a+ dendritic cells generated ex vivo from monocytes or hematopoietic precursor cells are CD304+ but CD303 (BDCA-2)–. | Additional information: Clone REA774 displays negligible binding to Fc receptors.
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文献和实验该产品被引用文献
Grabbe, S. et al. (2000) Dendritic cells: multi-lineal and multi-functional. Immunol. Today 21: 431–433. | Dzionek, A. et al. (2000) BDCA-2, BDCA-3, BDCA-4: Three markers for distinct subsets of dendritic cells in human peripheral blood. J Immunol 165: 6037–6046. | Dzionek, A. et al. (2001) BDCA-2, a novel plasmacytoid dendritic cell–specific type II C-type lectin, mediates antigen capture and is a potent inhibitor of interferon α/β induction. J. Exp. Med. 194: 1823–1834. | Dzionek, A. et al. (2002) Plasmacytoid dendritic cells: from specific surface markers to specific cellular functions. Hum. Immunol. 63: 1133–1148. | Jongbloed, S. L. et al. (2010) Human CD141+ (BDCA-3)+ dendritic cells (DCs) represent a unique myeloid DC subset that cross-presents necrotic cell antigens. J. Exp. Med. 207 (6): 1247–1260. | Mittag, D. et al. (2011) Human dendritic cell subsets from spleen and blood are similar in phenotype and function but modified by donor health status. J Immunol 186 (11): 6207–6217. | Jardine, L. et al. (2013) Rapid detection of dendritic cell and monocyte disorders using CD4 as a lineage marker of the human peripheral blood antigen-presenting cell compartment. Front Immunol 4: 495.
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CD304 (BDCA-4) Antibody, anti-human, Biotin, REAfinity™, 30 tests in 60 µL
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