- 询价
- Miltenyi Biotec
- 德国
- 130-119-166
- 2025年12月09日
- Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry
- human cell line
- mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
CD29
- 亚型：
recombinant human IgG1
- 形态：
Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
- 保存条件：
避光，4-8℃
- 克隆性：
REA1074
- 标记物：
APC
- 适应物种：
mouse, rat
- 保质期：
24个月
- 供应商：
Miltenyi Biotec
- 宿主：
human cell line
- 应用范围：
Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry
- 浓度：
1:50
- 抗体英文名：
CD29 Antibody, anti-mouse/rat, REAfinity™
- 抗体名：
CD29 Antibody, anti-mouse/rat, REAfinity™
- 规格：
30 µg in 200 µL
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文献和实验30s，接着40个循环的94℃ 15 s，65℃ 20 s，68℃ 5 min]，其中使用了1单位的ELONGASE酶混合物[PCR 50ml终体积，其中60 mM Tris－SO4（pH9.1），18 mM (NH4)2SO4，2 mM MgSO4，dNTP每个200 µM，引物每个400 nM ]，其中分别是AAP和引物TSC－2－2826（A带）或引物TSC－2－1732（B带）一起使用。每个PCR产物（带1和带3）取10µl 在0.8％琼脂糖上，1倍TAE凝胶电泳和SYBRò绿染色（分子
Coating of Platelets with Antibody in vitro
or an Ab directed to platelet Ag (you may use Grener F-bottom microtitration plate for this step). If the Ab used is rat IgG1 (k) against mouse CD41 (MWReg30, Becton Dickinson, Cat.: 09911D) then the advised concentration is 0.6µg/ml (0.15µg per 200µl
Immunofluorescent Staining of Mouse and Rat Leukocytes
(or 50 µl wash buffer for negative controls). Mix by gently vortexing or tapping. Incubate at 4°C for 20-40 min in the dark. Wash 2X with 200 µl wash buffer (or 3X if a biotin-conjugated primary antibody is used). After each centrifugation, 350 x g
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