- 询价
- Miltenyi Biotec
- 德国
- 130-129-770
- 2025年12月09日
- Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry
- human cell line
- mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
CD25
- 亚型：
recombinant human IgG1
- 形态：
Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
- 保存条件：
避光，4-8℃
- 克隆性：
REA568
- 标记物：
APC-Vio 770
- 适应物种：
mouse
- 保质期：
24个月
- 供应商：
Miltenyi Biotec
- 宿主：
human cell line
- 应用范围：
Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry
- 浓度：
1:50
- 抗体英文名：
CD25 Antibody, anti-mouse, REAfinity™
- 抗体名：
CD25 Antibody, anti-mouse, REAfinity™
- 规格：
30 µg in 200 µL
Identification and enumeration of CD25+ cells by flow cytometryClone REA568 recognizes the mouse CD25 antigen, the α chain of the IL-2 receptor (IL-2R). IL-2Rα associates with the β chain (CD122) and the γ chain (CD132) to form the functional IL-2R complex. IL-2Rα is expressed on CD4+CD25+ regulatory T cells, activated T and B cells, and to a lesser extent on activated dendritic cells. It is also transiently expressed during T and B cell development. Binding of the CD25 antibody does not impede the binding of IL-2 to its receptor. In combination with CD4, CD25 antibody can be used to identify regulatory T cells (Tregs). | Additional information: Clone REA568 displays negligible binding to Fc receptors. |
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文献和实验该产品被引用文献
Miller, J. et al. (1985) Nucleotide sequence and expression of a mouse interleukin 2 receptor cDNA. J Immunol 134 (6): 4212–4217. | Read, S. et al. (2000) Cytotoxic T lymphocyte-associated antigen 4 plays an essential role in the function of CD25+CD4+ regulatory cells that control intestinal inflammation. J. Exp. Med. 192 (2): 295–302. | Taniguchi, T. et al. (1993) The IL-2/IL-2 receptor system: a current overview. Cell 73 (1): 5–8.
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CD25 Antibody, anti-mouse, APC-Vio® 770, REAfinity™, 30 µg in 200 µL
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