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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
CD172a (SIRPα)
- 亚型:
recombinant human IgG1
- 形态:
Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
- 保存条件:
避光,4-8℃
- 克隆性:
REA490
- 标记物:
Biotin
- 适应物种:
rat
- 保质期:
24个月
- 供应商:
Miltenyi Biotec
- 宿主:
human cell line
- 应用范围:
Flow cytometry
- 浓度:
1:50
- 抗体英文名:
CD172a (SIRPα) Antibody, anti-rat, REAfinity™
- 抗体名:
CD172a (SIRPα) Antibody, anti-rat, REAfinity™
- 规格:
30 µg in 200 µL
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文献和实验) is then used to detect promoter specific transcripts (pRNAs) with the biotin linked pull-down assay (Figure 2) (comment 5);3.From the 200µl elute 30µl should be saved as input and the remaining 170µl from the extracted genomic DNA (Qiagen™ DNeasy) is mixed
capture beads (Component A) and negative beads (Component B) by gently vortexing for 30 seconds before use. 2) Label a sample tube for each fluorochrome-conjugated rat or hamster antibody you are using, and add 1 drop of AbC™ RH capture beads
Immunofluorescent Staining of Mouse and Rat Leukocytes
(or 50 µl wash buffer for negative controls). Mix by gently vortexing or tapping. Incubate at 4°C for 20-40 min in the dark. Wash 2X with 200 µl wash buffer (or 3X if a biotin-conjugated primary antibody is used). After each centrifugation, 350 x g
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