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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
CD160
- 亚型:
recombinant human IgG1
- 形态:
Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
- 保存条件:
避光,4-8℃
- 克隆性:
REA669
- 标记物:
Vio Bright FITC
- 适应物种:
mouse
- 保质期:
24个月
- 供应商:
Miltenyi Biotec
- 宿主:
human cell line
- 应用范围:
Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry
- 浓度:
1:50
- 抗体英文名:
CD160 Antibody, anti-mouse, REAfinity™
- 抗体名:
CD160 Antibody, anti-mouse, REAfinity™
- 规格:
30 µg in 200 µL
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文献和实验实验步骤 1. Using the AbC™ Anti-Mouse Bead Kit 1) Completely resuspend the AbC™ capture beads (Component A) and negative beads (Component B) by gently vortexing for 30 seconds before use. 2) Label
:NuPAGE® 凝胶可在250V下跑24分钟 标准运行步骤 为了获得有效的分离,传统Laemmli凝胶需要跑90分钟。相比之下,NuPAGE® 凝胶的标准步骤则为200V,35分钟。此外,如果使用加速步骤,仅需24分钟就能获得有效分离(250V)。 图2. 加快运行时间,而不牺牲性能。NuPAGE® 样品上样:(1) Mark12™ 未染色标准品,10 μL;(2) 大鼠肝裂解液,10 μg;(3) 溶菌酶,6 μg;(4) Bio
Dynabeads® Mouse T-Activator CD3/CD28 – for physiological activation of mouse T cells
volume of Dynabeads taken from the vial (step 2). 2. Activation of Mouse T Cells 1) Start with 8 × 104 purified T cells in 100-200 µl medium in a 96-well tissue culture plate. 2) Add 2 µl Dynabeads Mouse T-Activator CD3/CD
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