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CD16 Antibody, anti-human, Vio

® Bright B515, REAfinity™, 100 tests in 200 µL
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  • 询价
  • Miltenyi Biotec已认证
  • 德国
  • 130-119-616
  • 2025年12月09日
  • Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry, Mass cytometry
  • human cell line
  • human, non-human primate
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  • 企业认证

    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      CD16

    • 亚型

      recombinant human IgG1

    • 形态

      Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.

    • 保存条件

      避光,4-8℃

    • 克隆性

      REA423

    • 标记物

      Vio Bright B515

    • 适应物种

      human, non-human primate

    • 保质期

      24个月

    • 供应商

      Miltenyi Biotec

    • 宿主

      human cell line

    • 应用范围

      Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry, Mass cytometry

    • 浓度

      1:50

    • 抗体英文名

      CD16 Antibody, anti-human, REAfinity™

    • 抗体名

      CD16 Antibody, anti-human, REAfinity™

    • 规格

      100 tests in 200 µL

    Detection of human or non-human primate CD16+ cellsClone REA423 recognizes the human CD16 antigen, a single-pass type I membrane protein which is also known as low affinity immunoglobulin gamma Fc region receptor III (FcRIII). CD16 is a 50–80 kDa glycoprotein that is expressed in two different isoforms. The transmembrane form is found on human NK cells, macrophages, and mast cells, while the glycosylphosphatidylinositol (GPI)-linked form is present on neutrophils. The human CD16 antigen is a low-affinity receptor for aggregated IgG. The transmembrane form plays a role in signal transduction, NK cell activation, and antibody-dependent cellular cytotoxicity. Clone REA423 recognizes both the extracellular domain of the transmembrane form as well as the GPI-linked form of the human CD16 antigen. CD16 is expressed on the majority of rhesus monkey NK cells and on a subset of monocytes but not on granulocytes. | Additional information: Clone REA423 displays negligible binding to Fc receptors.

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    图标文献和实验
    该产品被引用文献
    Romee, R. et al. (2013) NK cell CD16 surface expression and function is regulated by a disintegrin and metalloprotease-17 (ADAM17). Blood 121 (18): 3599–3608. | Ravetch, J. V. et al. (1989) Alternative membrane forms of Fc gamma RIII(CD16) on human natural killer cells and neutrophils. Cell type-specific expression of two genes that differ in single nucleotide substitutions. J. Exp. Med. 170 (2): 481–497. | Ziegler-Heitbrock, L. (2007) The CD14+ CD16+ blood monocytes: their role in infection and inflammation. J Leukoc Biol 584 (592): 584–592. | Croci, S. et al. (2018) Higher frequencies of lymphocytes expressing the natural killer group 2D receptor in patients with Behçet disease. Front Immunol 9: 2157. | Veluchamy, J. et al. (2017) In vivo efficacy of umbilical cord blood stem cell-derived NK cells in the treatment of metastatic colorectal cancer. Front Immunol 8: 87.
    相关实验
    • Isolation of Untouched Human B Cells by Depletion of Non-B Cells

      in the same volume of Isolation Buffer as the initial volume of Dynabeads ®(step 2). 2.        Isolation Procedure 1)        Transfer 500 μl (5 × 107 ) PBMC in Isolation Buffer to a tube. 2)        Add 100 μl of Antibody Mix. 3)        Mix

    • AbC Anti-Rat/Hamster Bead Kit

      1 μL   5)      Label another sample tube for each fluorochrome-conjugated antibody you are using, and add 1 drop of AbC capture beads (Component A in the AbC Anti-Mouse Bead Kit) to each tube. 6)     

    • AbC Anti-Mouse Bead Kit

      实验步骤   1.  Using the AbC Anti-Mouse Bead Kit 1)        Completely resuspend the AbC capture beads (Component A) and negative beads (Component B) by gently vortexing for 30 seconds before use. 2)        Label

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    CD16 Antibody, anti-human, Vio® Bright B515, REAfinity™, 100 tests in 200 µL
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