- 询价
- Miltenyi Biotec
- 德国
- 130-111-592
- 2025年12月09日
- Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry
- human cell line
- human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
CD117
- 亚型：
recombinant human IgG1
- 形态：
Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
- 保存条件：
避光，2-8℃
- 克隆性：
REA787
- 标记物：
PE
- 适应物种：
human
- 保质期：
24个月
- 供应商：
Miltenyi Biotec
- 宿主：
human cell line
- 应用范围：
Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry
- 浓度：
1:50
- 抗体英文名：
CD117 Antibody, anti-human, REAfinity™
- 抗体名：
CD117 Antibody, anti-human, REAfinity™
- 规格：
100 tests in 200 µL
Identification and enumeration of CD117+ cells by flow cytometryClone REA787 recognizes the human CD117 antigen, also known as c-kit or SCF receptor, which is a 145 kDa receptor tyrosine kinase that is involved in stem cell signaling, activation, and proliferation. The CD117 antigen is expressed on about 1–3% of mononuclear cells from peripheral blood mononuclear cells (PBMCs) or cord blood and on up to 10% of bone marrow cells. CD117 is further expressed on basophils, myeloid dendritic cells, TCRα/β+ T cells, CD19+ B cells, and CD56+ NK cells, as well as on mast cells, melanocytes, and acute myeloid leukemia (AML) blasts. Clone REA787 recognizes a different epitope than clone AC126 and may interfere with SCF-binding. It is therefore not recommended for indirect cell isolation, but is optimal for flow cytometric analysis of cells separated with the CD117 MicroBead Kit, human. | Additional information: Clone REA787 displays negligible binding to Fc receptors.
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文献和实验该产品被引用文献
Bühring, H. J. et al. (1991) The product of the proto-oncogene c-kit (P145c-kit) is a human bone marrow surface antigen of hematopoietic precursor cells which is expressed on a subset of acute non-lymphoblastic cells. Leukemia 5: 854–860. | Kubo, H. et al. (2008) Increased cardiac myocyte progenitors in failing human hearts. Circulation 118: 649–657. | Berglund, E. et al. (2013) Evidence for Ca2+-regulated ATP release in gastrointestinal stromal tumors. Exp. Cell Res. 319 (8): 1229–1238.
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CD117 Antibody, anti-human, PE, REAfinity™, 100 tests in 200 µL
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