- 询价
- Miltenyi Biotec
- 德国
- 130-116-220
- 2026年06月02日
- Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry
- human cell line
- mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
CD106 (VCAM-1)
- 亚型：
recombinant human IgG1
- 形态：
Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
- 保存条件：
避光，2-8℃
- 克隆性：
REA971
- 标记物：
PE
- 适应物种：
mouse
- 保质期：
24个月
- 供应商：
Miltenyi Biotec
- 宿主：
human cell line
- 应用范围：
Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry
- 浓度：
1:50
- 抗体英文名：
CD106 (VCAM-1) Antibody, anti-mouse, REAfinity™
- 抗体名：
CD106 (VCAM-1) Antibody, anti-mouse, REAfinity™
- 规格：
150 µg in 1 mL
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文献和实验Immunofluorescent Staining of Mouse and Rat Leukocytes
16 (Mouse BD Fc Block™; Cat No. 553141/553142) or anti-rat CD32 (Rat BD Fc Block™; Cat. No. 550270/550271) is recommended. BD Fc Block™ can be added to cells (~0.25 µg per million cells, 3 - 5 min, 4°C) and need not be washed out prior to addition of primary
Phycoerythrin conjugation protocol
1 ml purified antibody at approx 2.5 mg/ml in PBS. Antibody must be fairly pure - i.e. purify on protein A or G column before conjugating. Add 20 µl SMCC, 1.7 mg/ml in DMSO. Incubate for 1 hour. Take product from step (2) and add
with the better the chances of a successful pulldown. A total of 4.0×106 293 HEK cells (~70% confluent) are plated and 24 hours later transfected with 15µg of a RNA Polymerase II expressing flag-tagged construct plasmid. We generally use Lipofectamine 2000™
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