- 询价
- Miltenyi Biotec
- 德国
- 130-114-511
- 2025年12月09日
- Intracellular flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry
- human cell line
- human, mouse, rat, non-human primate, other
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
PCNA
- 亚型：
recombinant human IgG1
- 形态：
Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
- 保存条件：
避光，2-8℃
- 克隆性：
REA858
- 标记物：
FITC
- 适应物种：
human, mouse, rat, non-human primate, other
- 保质期：
24个月
- 供应商：
Miltenyi Biotec
- 宿主：
human cell line
- 应用范围：
Intracellular flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry
- 浓度：
1:50
- 抗体英文名：
PCNA Antibody, anti-human/mouse/rat, REAfinity™
- 抗体名：
PCNA Antibody, anti-human/mouse/rat, REAfinity™
- 规格：
100 tests in 200 µL
Identification and enumeration of PCNA+ cells by flow cytometryClone REA858 recognizes the human, mouse, and rat proliferating cell nuclear antigen (PCNA), a 36 kDa nuclear protein also known as cyclin or auxiliary protein of DNA polymerase delta. PCNA is expressed in the nucleus of all proliferating cells. PCNA plays a role in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Furthermore, it is involved in DNA damage response by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. | Additional information: Clone REA858 displays negligible binding to Fc receptors.
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文献和实验该产品被引用文献
Landberg, G. et al. (1990) Flow cytometric multiparameter analysis of proliferating cell nuclear antigen/cyclin and Ki-67 antigen: a new view of the cell cycle. Exp. Cell Res. 187 (1): 111–118. | Guzińska-Ustymowicz, K. et al. (2009) Correlation between proliferation markers: PCNA, Ki-67, MCM-2 and antiapoptotic protein Bcl-2 in colorectal cancer. Anticancer Res. 29 (8): 3049–3052. | Cazzalini, O. et al. (2014) CBP and p300 acetylate PCNA to link its degradation with nucleotide excision repair synthesis. Nucleic Acids Res. 42 (13): 8433–8448. | Ortega, J. et al. (2015) Phosphorylation of PCNA by EGFR inhibits mismatch repair and promotes misincorporation during DNA synthesis. Proc. Natl. Acad. Sci. U.S.A. 112 (18): 5667–5672.
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PCNA Antibody, anti-human/mouse/rat, FITC, REAfinity™, 100 tests in 200 µL
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