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PAX-5 (BSAP) Antibody, anti-hu

man/mouse, APC, REAfinity™, 30 tests in 60 µL
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  • 询价
  • Miltenyi Biotec已认证
  • 德国
  • 130-131-961
  • 2025年12月09日
  • Intracellular flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry
  • human cell line
  • human, mouse
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      PAX-5 (BSAP)

    • 亚型

      recombinant human IgG1

    • 形态

      Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.

    • 保存条件

      避光,2-8℃

    • 克隆性

      REA140

    • 标记物

      APC

    • 适应物种

      human, mouse

    • 保质期

      24个月

    • 供应商

      Miltenyi Biotec

    • 宿主

      human cell line

    • 应用范围

      Intracellular flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry

    • 浓度

      1:50

    • 抗体英文名

      PAX-5 (BSAP) Antibody, anti-human/mouse, REAfinity™

    • 抗体名

      PAX-5 (BSAP) Antibody, anti-human/mouse, REAfinity™

    • 规格

      30 tests in 60 µL

    Detection of human or mouse PAX-5+ cellsClone REA140 recognizes PAX-5, which belongs to the paired box (PAX) gene family of transcription factors. PAX-5 is the only mammalian PAX transcription factor, which is expressed in hematopoietic cells. Within the hematopoietic cells, expression of PAX-5 is limited to cells of the B-lymphocyte lineage and it exerts transcriptional control of B cell commitment, development, and tumorigenesis. PAX-5 contains DNA binding conserved ‘paired’ domain, which consists of an N- and a C-terminal subdomain. Each subregion contains a homeodomain-like helix-turn-helix motif, which binds to a half-site of the PAX-5 recognition sequence. Depending upon the interaction partners, PAX-5 serves either as an activator or repressor of transcription. For instance, Groucho protein family members, by binding to a conserved octapeptide motif of PAX-5, enable PAX-5 to serve as a repressor. | Additional information: Clone REA140 displays negligible binding to Fc receptors. |

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    图标文献和实验
    该产品被引用文献
    Pridans, C. et al. (2008) Identification of Pax5 target genes in early B cell differentiation. J Immunol 180: 1719–1728. | Fazio, G. et al. (2008) PAX5/TEL acts as a transcriptional repressor causing down-modulation of CD19, enhances migration to CXCL12, and confers survival advantage in pre-BI cells. Cancer Res. 68: 181–189. | He, T. et al. (2011) Histone acetyltransferase p300 acetylates Pax5 and strongly enhances Pax5-mediated transcriptional activity. J. Biol. Chem. 286: 14137–14145.
    相关实验
    • RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

         3.75ml 4M NaCl       3ml 10% Triton X (Tx-100)       300µl 10% NAD       21.45ml H20 ChIP wash buffer       30ml final volume:       150µl 2M Tris       1.7ml 4.4M LiCl       60µl 0.5M EDTA       1.5ml 10% NAD       1.5ml 10% NP-40       25.09ml

    • Cell Surface Immunofluorescence Staining Protocol

      -CD4-Biotin, and anti-CD8-APC) and incubate on ice for 15-20 minutes in the dark. 9) Wash 2X with at least 2 ml of Cell Staining Buffer by centrifugation at 350 x g for 5 minutes. 1) If using a purified primary antibody, resuspend

    • 在无血清细胞培养条件下将人 iPS 细胞体外分化为结肠类器官

      C下265 x g离心5分钟。 吸出上清液并用 4 mL 1X PBS 洗涤一次。 4 °C下265 x g离心5分钟并吸出上清液。 轻弹锥形管的底部,使沉淀物脱落。 将 ECM基质胶置于 TC 操作台中,快速取出 1 mL 并将其添加到步骤 16 中脱落的细胞沉淀中。 用设置为 900 µL 的 P-1000 移液管在 ECM 基质胶中轻轻重悬细胞沉淀。 避免在在悬浮过程中引入气泡。 立即将细胞悬液置于冰上 5 分钟以防止凝胶化。 以1:50-1:100 将 1 mL ECM基质

    图标技术资料

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    文献支持
    PAX-5 (BSAP) Antibody, anti-human/mouse, APC, REAfinity™, 30 tests in 60 µL
    询价