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Oct3/4 Antibody, anti-human/mo

use, APC, REAfinity™, 100 tests in 200 µL
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  • 询价
  • Miltenyi Biotec已认证
  • 德国
  • 130-123-257
  • 2025年12月09日
  • Intracellular flow cytometry
  • human cell line
  • human, mouse
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      Oct3/4

    • 亚型

      recombinant human IgG1

    • 形态

      Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.

    • 保存条件

      避光,2-8℃

    • 克隆性

      REA622

    • 标记物

      APC

    • 适应物种

      human, mouse

    • 保质期

      24个月

    • 供应商

      Miltenyi Biotec

    • 宿主

      human cell line

    • 应用范围

      Intracellular flow cytometry

    • 浓度

      1:50

    • 抗体英文名

      Oct3/4 Antibody, anti-human/mouse, REAfinity™

    • 抗体名

      Oct3/4 Antibody, anti-human/mouse, REAfinity™

    • 规格

      100 tests in 200 µL

    Identification and enumeration of Oct3/4+ cells by flow cytometryClone REA622 recognizes the human and mouse octamer-binding transcription factor 3/4 (Oct3/4). Oct3/4 is a homeodomain transcription factor belonging to the POU transcription factor family and is also known as POU domain class 5 transcription factor 1 (POU5F1). Oct3/4 is critical for the maintenance of totipotency in primate blastomeres and for pluripotency of the inner cell mass of developing mammalian embryos. This critical role of Oct3/4 is maintained in embryonic stem cells, the developing germline, and germline tumors, such as seminomas and embryonic teratocarcinomas. When inducing pluripotency in differentiated cells, Oct3/4 appears to be an essential but not the only required transcription factor. Oct3/4 acts as a transcriptional activator of genes involved in maintenance of undifferentiated state and as a repressor of differentiation-specific genes. | Additional information: Clone REA622 displays negligible binding to Fc receptors.

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    图标文献和实验
    该产品被引用文献
    Nakagawa, E. et al. (2012) The novel function of Oct3/4 in mouse tooth development. Histochem. Cell Biol. 137 (3): 367–376. | Niwa, H. et al. (2000) Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells. Nat. Genet. 24 (4): 372–376. | Wey, E. et al. (1994) A human POU domain gene, mPOU, is expressed in developing brain and specific adult tissues. Eur. J. Biochem. 220 (3): 753–762.
    相关实验
    • APC和TSC-2 cDNA的长5'RACE

       图2 APC 5'末端的扩增。用对应APC mRNA的位置5846的GSP反转录1mg Hela总RNA得到APC mRNA。cDNA被纯化、加尾,和用对应位置1797(A带)和位置1632(B带)的引物和AAP一起,用ELONGASE酶混合物来扩增(94℃ 30s,接着35个循环的94℃ 15 s,55℃ 30 s,68℃ 5 min)。初步PCR用TE缓冲液稀释100倍,取1ml用引物APC-1632(A带)或引物APC-1242(B带)和AUAP分别重新扩增(30个循环),每个PCR

    • (共享)ELISA原理与实验方法

      , incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS

    • Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum

      with deionized water, and mix Mouse osteocalcin standard, one vial, 200 ng, lyophilized: reconstitute standard with exactly 2.0 ml of sample buffer (yields a 100 ng/ml solution); use

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    文献支持
    Oct3/4 Antibody, anti-human/mouse, APC, REAfinity™, 100 tests in 200 µL
    询价