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MRP14 Antibody, anti-human, AP

C, REAfinity™, 100 tests in 200 µL
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  • 询价
  • Miltenyi Biotec已认证
  • 德国
  • 130-114-517
  • 2025年12月09日
  • Intracellular flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry
  • human cell line
  • human
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      MRP14

    • 亚型

      recombinant human IgG1

    • 形态

      Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.

    • 保存条件

      避光,2-8℃

    • 克隆性

      REA859

    • 标记物

      APC

    • 适应物种

      human

    • 保质期

      24个月

    • 供应商

      Miltenyi Biotec

    • 宿主

      human cell line

    • 应用范围

      Intracellular flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry

    • 浓度

      1:50

    • 抗体英文名

      MRP14 Antibody, anti-human, REAfinity™

    • 抗体名

      MRP14 Antibody, anti-human, REAfinity™

    • 规格

      100 tests in 200 µL

    Identification and enumeration of MRP14+ cells by flow cytometryClone REA859 recognizes the human MRP14 antigen, a calcium- and zinc-binding protein also known as S100A9 or calgranulin-B. MRP14 is mainly expressed in myeloid cells, e.g. MDSCs, neutrophils, and monocytes, and plays an important role in the regulation of inflammatory processes and immune response. MRP14 is involved in neutrophil chemotaxis and adhesion, and is able to increase the bactericidal activity of neutrophils. | Additional information: Clone REA859 displays negligible binding to Fc receptors.

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    图标文献和实验
    该产品被引用文献
    Odink, K. et al. (1987) Two calcium-binding proteins in infiltrate macrophages of rheumatoid arthritis. Nature 330 (6143): 80–82. | Rammes, A. et al. (1997) Myeloid-related protein (MRP) 8 and MRP14, calcium-binding proteins of the S100 family, are secreted by activated monocytes via a novel, tubulin-dependent pathway. J. Biol. Chem. 272 (14): 9496–9502. | Loser, K. et al. (2010) The toll-like receptor 4 ligands Mrp8 and Mrp14 are crucial in the development of autoreactive CD8+ T cells. Nat Med 16 (6): 713–717.
    相关实验
    • APC和TSC-2 cDNA的长5'RACE

      30s,接着40个循环的94℃ 15 s,65℃ 20 s,68℃ 5 min],其中使用了1单位的ELONGASE酶混合物[PCR 50ml终体积,其中60 mM Tris-SO4(pH9.1),18 mM (NH4)2SO4,2 mM MgSO4,dNTP每个200 µM,引物每个400 nM ],其中分别是AAP和引物TSC-2-2826(A带)或引物TSC-2-1732(B带)一起使用。每个PCR产物(带1和带3)取10µl 在0.8%琼脂糖上,1倍TAE凝胶电泳和SYBRò绿染色(分子

    • AbC Anti-Mouse Bead Kit

      suspension. 5)        Label another sample tube for each fluorochrome-conjugated antibody you are using, and add 1 drop of AbC capture beads (Component A in the AbC Anti-Mouse Bead Kit) to each tube. 6)        Add a pre-titrated

    • AbC Anti-Rat/Hamster Bead Kit

      1 μL   5)      Label another sample tube for each fluorochrome-conjugated antibody you are using, and add 1 drop of AbC capture beads (Component A in the AbC Anti-Mouse Bead Kit) to each tube. 6)     

    图标技术资料

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    文献支持
    MRP14 Antibody, anti-human, APC, REAfinity™, 100 tests in 200 µL
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