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Ly-49D Antibody, anti-mouse, A

PC, REAfinity™, 150 µg in 1 mL
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  • 询价
  • Miltenyi Biotec已认证
  • 德国
  • 130-117-163
  • 2025年12月09日
  • Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry
  • human cell line
  • mouse
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      Ly-49D

    • 亚型

      recombinant human IgG1

    • 形态

      Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.

    • 保存条件

      避光,2-8℃

    • 克隆性

      REA1020

    • 标记物

      APC

    • 适应物种

      mouse

    • 保质期

      24个月

    • 供应商

      Miltenyi Biotec

    • 宿主

      human cell line

    • 应用范围

      Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry

    • 浓度

      1:50

    • 抗体英文名

      Ly-49D Antibody, anti-mouse, REAfinity™

    • 抗体名

      Ly-49D Antibody, anti-mouse, REAfinity™

    • 规格

      150 µg in 1 mL

    Identification and enumeration of Ly-49D+ cells by flow cytometryClone REA1020 recognizes Ly-49D, a type II transmembrane NK cell receptor belonging to the Ly-49 family. Members of the Ly-49 family are homodimeric C-type lectin receptors, recognizing MHC class I molecules as ligands, and include activating and inhibitory receptor molecules. Ly-49D is a prototypic activating member of the Ly-49 family and in contrast to inhibitory members, lacks a cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). A positive signal is initiated by interaction of Ly-49D with immunoreceptor tyrosine-based activating motif (ITAM) containing transmembrane adapter DAP12. Expression of Ly-49D is strain restricted and is found on subsets of NK cells in C57BL/6, C3H/He, and SJL but not in BALB/c, DBA/2, AKR, and CBA/J mice. | Additional information: Clone REA1020 displays negligible binding to Fc receptors.

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    图标文献和实验
    该产品被引用文献
    Yokoyama, W. M. et al. (1993) The Ly-49 and NKR-P1 gene families encoding lectin-like receptors on natural killer cells: the NK gene complex. Annu. Rev. Immunol. 11: 613–635. | Mason, L. H. et al. (1996) The Ly-49D receptor activates murine natural killer cells. J. Exp. Med. 184: 2119–2128. | Hanke, T. et al. (1999) Direct assessment of MHC class I binding by seven Ly49 inhibitory NK cell receptors. Immunity 11 (1): 67–77.
    相关实验
    • AbC Anti-Mouse Bead Kit

      suspension. 5)        Label another sample tube for each fluorochrome-conjugated antibody you are using, and add 1 drop of AbC capture beads (Component A in the AbC Anti-Mouse Bead Kit) to each tube. 6)        Add a pre-titrated

    • IMMUNOSCREENING OF WITH ANTIBODY PROBES

      in 10mM IPTG (MW=238.31). Briefly blot the excess solution from the filters, and wrap them in cellophane. These should be moist and stored at 4°C. We typically adsorb serum with five 150 mm Petri dishes of lysed E. coli to reduce anti-E. coli antibody

    • Anti-HA Immunocytology

      -purified goat anti-mouse IgG (Jackson Labs; 1 mg/ml stock used at 1:300 dilution) Texas Red-conjugated affinity-purified donkey anti-rabbit (Jackson Labs;1 mg/ml stock used at 1:200 dilution)  

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    文献支持
    Ly-49D Antibody, anti-mouse, APC, REAfinity™, 150 µg in 1 mL
    询价