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Ly-49A Antibody, anti-mouse, P

E-Vio® 770, REAfinity™, 30 µg in 200 µL
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  • 询价
  • Miltenyi Biotec已认证
  • 德国
  • 130-117-115
  • 2025年12月09日
  • Flow cytometry
  • human cell line
  • mouse
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      Ly-49A

    • 亚型

      recombinant human IgG1

    • 形态

      Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.

    • 保存条件

      避光,2-8℃

    • 克隆性

      REA1018

    • 标记物

      PE-Vio 770

    • 适应物种

      mouse

    • 保质期

      24个月

    • 供应商

      Miltenyi Biotec

    • 宿主

      human cell line

    • 应用范围

      Flow cytometry

    • 浓度

      1:50

    • 抗体英文名

      Ly-49A Antibody, anti-mouse, REAfinity™

    • 抗体名

      Ly-49A Antibody, anti-mouse, REAfinity™

    • 规格

      30 µg in 200 µL

    Identification and enumeration of Ly-49A+ cells by flow cytometry.Clone REA1018 recognizes th mouse Ly-49A antigen, an inhibitory cell surface receptor belonging to the Ly-49 receptor family. Ly-49A is a 44 kDa type II transmembrane, disulfide-linked homodimer with C-type lectin extracellular domains. Expression of Ly-49A is found on subset of NK and NK-T cells. Ly-49 family members are both activating and inhibitory receptors and are mainly receptors for MHC class I molecules. Ly-49A interacts strongly with MHC class Ia molecules such as H-2Dd, H-2Dk, and weakly with H-2Db. An inhibitory signal is delivered by Ly-49A engagement via the phosphorylation of immunoreceptor tyrosine based inhibition motif (ITIM) and recruitment of phosphatases SHP-1 and SHP-2. Recognition of cognate ligand bearing cells leads to inhibition of cytotoxic killing of cells by NK cells. | Additional information: Clone REA1018 displays negligible binding to Fc receptors.

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    图标文献和实验
    该产品被引用文献
    Daws, M. R. et al. (1999) H-2Dd engagement of Ly49A leads directly to Ly49A phosphorylation and recruitment of SHP1. Immunology 97 (4): 656–664. | Chung, D. H. et al. (2000) Mapping the ligand of the NK inhibitory receptor Ly49A on living cells. J Immunol 165: 6922–6932. | Zimmer, J. et al. (2001) H-2D ligand expression by Ly49A+ natural killer (NK) cells precludes ligand uptake from environmental cells: Implications for NK cell function. J. Exp. Med. 194 (10): 1531–1539.
    相关实验
    • AbC Anti-Mouse Bead Kit

      实验步骤   1.  Using the AbC Anti-Mouse Bead Kit 1)        Completely resuspend the AbC capture beads (Component A) and negative beads (Component B) by gently vortexing for 30 seconds before use. 2)        Label

    • Monoclonal Antibody Production

      drip off the end of the tail until you have collected ~200 µl.(You may have to pre-bleed twice,with a week or so between bleeds). (5) Take the collected blood and place at 37 ℃ for 30 min.to remove complement. (6) Place blood at 4 ℃ overnight to clot

    • Anti-GFP/BrdU In Situ Immunostaining

      Dilute 1 µL fresh streptavidin-Alexa Flour 555 in 1 mL PBS (1:1,000).  Spin the dilute antibody at high speed for 30'  to remove particulates.  Add supernatant to specimen and incubate 30' at room temp.  Remove antibody and add 1 mL PBS.  O.K

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    文献支持
    Ly-49A Antibody, anti-mouse, PE-Vio® 770, REAfinity™, 30 µg in 200 µL
    询价