Ly-49I Antibody, anti-mouse, P

Identification and enumeration of Ly-49I+ cells by flow cytometryClone REA1043 recognizes the mouse Ly49I antigen, a single-pass type II C-type lectin-like membrane glycoprotein also known as Ly49 inhibitory receptor I. The Ly49 receptors are encoded by a family of highly polymorphic and polygenic genes within the mouse NK gene complex which includes genes that encode both inhibitory and activating Ly49 receptors. Ly49 receptors recognize MHC-I and MHC-I–like proteins on normal as well as on altered cells. They are described as being expressed on both the developing and mature NK cells. The inhibitory Ly49 receptors are involved in NK cell education, a process in which NK cells acquire function and tolerance toward cells that express “self-MHC-I.” On the other hand, the activating Ly49 receptors recognize altered cells expressing activating ligands. Ly49 receptors have been described on multiple NK cell subsets, such as uterine NK and memory NK cells, as well as NKT cells, dendritic cells, plasmacytoid dendritic cells, macrophages, neutrophils, and cells of the adaptive immune system, such as activated T cells and regulatory CD8+ T cells. Ly49I is an inhibitory receptor capable of down-modulating proliferation, IFN-γ response, and cytotoxic activity in cells that express it. | Additional information: Clone REA1043 displays negligible binding to Fc receptors.