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IRF-7 Antibody, anti-human, AP

C, REAfinity™, 30 tests in 300 µL
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  • 询价
  • Miltenyi Biotec已认证
  • 德国
  • 130-108-906
  • 2025年12月09日
  • Intracellular flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry
  • human cell line
  • human
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  • 企业认证

    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      IRF-7

    • 亚型

      recombinant human IgG1

    • 形态

      Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.

    • 保存条件

      避光,2-8℃

    • 克隆性

      REA521

    • 标记物

      APC

    • 适应物种

      human

    • 保质期

      24个月

    • 供应商

      Miltenyi Biotec

    • 宿主

      human cell line

    • 应用范围

      Intracellular flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry

    • 浓度

      1:11

    • 抗体英文名

      IRF-7 Antibody, anti-human, REAfinity™

    • 抗体名

      IRF-7 Antibody, anti-human, REAfinity™

    • 规格

      30 tests in 300 µL

    Identification and enumeration of IRF-7+ cells by flow cytometryClone REA521 recognizes the human interferon regulatory factor 7 (IRF-7) antigen, regardless of phosphorylation status. IRF-7 is a member of the IRF family of transcription factors and a key player in the innate immune response against viral infections. Constitutive expression of IRF-7 is limited to peripheral blood lymphocytes and dendritic cells while in most cell types its expression can be induced by type I interferon. IRF-7 is sequestered in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA, or toll-like receptor signaling, it becomes phosphorylated by TBK and IKK-i kinases. Phosphorylated IRF-7 migrates in the nucleus where it can activate interferon type I genes and other interferon-stimulated genes. | Additional information: Clone REA521 displays negligible binding to Fc receptors.

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    图标文献和实验
    该产品被引用文献
    Sgarbanti, M. et al. (2007) IRF-7: new role in the regulation of genes involved in adaptive immunity. Ann. N. Y. Acad. Sci. 1095: 325–333. | Smith, E. J. et al. (2001) IRF3 and IRF7 phosphorylation in virus-infected cells does not require double-stranded RNA-dependent protein kinase R or Ikappa B kinase but is blocked by Vaccinia virus E3L protein. J. Biol. Chem. 276 (12): 8951–8957. | Zhang, L. and Pagano, J. S. et al. (1997) IRF-7, a new interferon regulatory factor associated with Epstein-Barr virus latency. Mol. Cell. Biol. 17 (10): 5748–5757.
    相关实验
    • AbC Anti-Mouse Bead Kit

      实验步骤   1.  Using the AbC Anti-Mouse Bead Kit 1)        Completely resuspend the AbC capture beads (Component A) and negative beads (Component B) by gently vortexing for 30 seconds before use. 2)        Label

    • AbC Anti-Rat/Hamster Bead Kit

      1 μL   5)      Label another sample tube for each fluorochrome-conjugated antibody you are using, and add 1 drop of AbC capture beads (Component A in the AbC Anti-Mouse Bead Kit) to each tube. 6)     

    • Anti-GFP/BrdU In Situ Immunostaining

      Dilute 1 µL fresh streptavidin-Alexa Flour 555 in 1 mL PBS (1:1,000).  Spin the dilute antibody at high speed for 30'  to remove particulates.  Add supernatant to specimen and incubate 30' at room temp.  Remove antibody and add 1 mL PBS.  O.K

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    文献支持
    IRF-7 Antibody, anti-human, APC, REAfinity™, 30 tests in 300 µL
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