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H-2Db Antibody, anti-mouse, AP

C, REAfinity™, 30 µg in 1 mL
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  • 询价
  • Miltenyi Biotec已认证
  • 德国
  • 130-109-608
  • 2025年12月09日
  • Flow cytometry
  • human cell line
  • mouse
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      H-2Db

    • 亚型

      recombinant human IgG1

    • 形态

      Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.

    • 保存条件

      避光,2-8℃

    • 克隆性

      REA619

    • 标记物

      APC

    • 适应物种

      mouse

    • 保质期

      24个月

    • 供应商

      Miltenyi Biotec

    • 宿主

      human cell line

    • 应用范围

      Flow cytometry

    • 浓度

      1:10

    • 抗体英文名

      H-2Db Antibody, anti-mouse, REAfinity™

    • 抗体名

      H-2Db Antibody, anti-mouse, REAfinity™

    • 规格

      30 µg in 1 mL

    Identification and enumeration of H-2Db+ cells by flow cytometryClone REA619 recognizes the mouse H-2Db MHC class I alloantigen expressed on mouse MHC class I+ cells of the H-2Db haplotype. Reactivity to other haplotypes like, for example, a, d, f, k, n, p, q, r, s, u, and v, has not been observed. | Additional information: Clone REA619 displays negligible binding to Fc receptors.

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    图标文献和实验
    该产品被引用文献
    Glithero, A. et al. (1999) Crystal structures of two H-2Db/glycopeptide complexes suggest a molecular basis for CTL cross-reactivity. Immunity 10 (1): 63–74. | Pascolo, S. et al. (1997) HLA-A2.1-restricted education and cytolytic activity of CD8+ T lymphocytes from beta2 microglobulin (beta2m) HLA-A2.1 monochain transgenic H-2Db beta2m double knockout mice. J. Exp. Med. 185 (12): 2043–2051. | van Stipdonk, M. J. et al. (2009) Design of agonistic altered peptides for the robust induction of CTL directed towards H-2Db in complex with the melanoma-associated epitope gp100. Cancer Res. 69 (19): 7784–7792.
    相关实验
    • APC和TSC-2 cDNA的长5'RACE

       图2 APC 5'末端的扩增。用对应APC mRNA的位置5846的GSP反转录1mg Hela总RNA得到APC mRNA。cDNA被纯化、加尾,和用对应位置1797(A带)和位置1632(B带)的引物和AAP一起,用ELONGASE酶混合物来扩增(94℃ 30s,接着35个循环的94℃ 15 s,55℃ 30 s,68℃ 5 min)。初步PCR用TE缓冲液稀释100倍,取1ml用引物APC-1632(A带)或引物APC-1242(B带)和AUAP分别重新扩增(30个循环),每个PCR

    • AbC Anti-Mouse Bead Kit

      实验步骤   1.  Using the AbC Anti-Mouse Bead Kit 1)        Completely resuspend the AbC capture beads (Component A) and negative beads (Component B) by gently vortexing for 30 seconds before use. 2)        Label

    • ANTIBODY BINDING TO PROTEIN A AND PROTEIN G

      of various immunoglobulines. Protein A is a 42 kDa polypeptide that binds the residues in the CH2 and CH3 domains of immunoglobulin heavy chains. Protein G is an approximatley 30 kDa polypeptide that binds immunoglobulins and albumin. Recombinant protein G

    图标技术资料

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    文献支持
    H-2Db Antibody, anti-mouse, APC, REAfinity™, 30 µg in 1 mL
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