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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
HA
- 亚型:
mouse IgG1
- 保存条件:
避光,2-8℃
- 克隆性:
GG8-1F3.3.1
- 标记物:
FITC
- 适应物种:
any
- 保质期:
24个月
- 供应商:
Miltenyi Biotec
- 宿主:
mouse
- 应用范围:
Flow cytometry, Intracellular flow cytometry, Western blotting, ELISA
- 浓度:
1:50
- 靶点:
HA
- 抗体英文名:
HA Antibody
- 抗体名:
HA Antibody
- 规格:
100 tests in 200 µL
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文献和实验孵育15分钟;(6)PI标记:上机前5分钟再加入5μL的PI染色。(7)上机前,补加200μL的1×Binding Buffer。 四、保存:4℃保存;避免反复冻融。 五、注意事项(1)本试剂只适用于实验,不适用于临床检测;(2)PI(propidium iodide,碘化丙锭)有毒性,能通过皮肤吸收,对眼睛有刺激作用,使用时需戴手套;(3)Annexin V-FITC和PI是光敏物质,在操作时请注意避光。在处理和标记时,尽可能在暗处进行。在孵育阶段,用铝箔包裹容器或置于抽屉中。细胞标记后,在暗
Monoclonal Antibody Production
drip off the end of the tail until you have collected ~200 µl.(You may have to pre-bleed twice,with a week or so between bleeds). (5) Take the collected blood and place at 37 ℃ for 30 min.to remove complement. (6) Place blood at 4 ℃ overnight to clot
Antibody Staining of Ovaries 1. Dissect ovaries in EBR 2. Transfer ovaries into an Eppendorf tube and add 100 µl devitellinizing buffer and 600-µl heptane 3. Agitate gently for 10 minutes
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