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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
c-Cbl pY774
- 亚型:
recombinant human IgG1
- 形态:
Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
- 保存条件:
避光,2-8℃
- 克隆性:
REA118
- 标记物:
Vio B515
- 适应物种:
human
- 保质期:
24个月
- 供应商:
Miltenyi Biotec
- 宿主:
human cell line
- 应用范围:
Intracellular flow cytometry
- 浓度:
1:50
- 靶点:
c-Cbl pY774
- 抗体英文名:
c-Cbl pY774 Antibody, anti-human, REAfinity™
- 抗体名:
c-Cbl pY774 Antibody, anti-human, REAfinity™
- 规格:
30 tests in 60 µL
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文献和实验Vybrant® DyeCycle Green and Orange Stains
degradation. Incubate at 37?C for 30 minutes, protected from light. For cells stained with Vybrant® DyeCycle™ Green stain, analyze samples on flow cytometer using 488 nm excitation and green emission (Figure 3). For cells stained with Vybrant®
A/B/C Random Amplification Protocol
tubes. Add 1 µL DNA (40 ng) to each tube. Digest DNA then heat inactivate enzyme on PCR machine: ( 37ºC x60', 65ºC x10') x1 (4ºC hold) 4. While the genomic DNA is digesting set up master mixes for Reactions I, II, and III
性(图1)。NuPAGE® 样品缓冲液的pH值不会低于pH 8(即使是加热到70°C),因此样品制备过程中蛋白样品不会降解。 图1. 看看NuPAGE® 凝胶的与众不同之处。(A) NuPAGE® 凝胶利用1×MES缓冲液运行。(B) tris-甘氨酸凝胶利用tris-甘氨酸/SDS缓冲液运行。图片显示,与tris-甘氨酸凝胶相比,NuPAGE® 凝胶产生锐利、完整的条带,代表了未水解的蛋白,特别是在第3、5、7、8和9道。 样品上样:(1) Mark12™
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