- 询价
- Miltenyi Biotec
- 德国
- 130-105-253
- 2025年12月09日
- Intracellular flow cytometry
- human cell line
- human, mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
c-Cbl pY700
- 亚型：
recombinant human IgG1
- 形态：
Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
- 保存条件：
避光，2-8℃
- 克隆性：
REA362
- 标记物：
APC
- 适应物种：
human, mouse
- 保质期：
24个月
- 供应商：
Miltenyi Biotec
- 宿主：
human cell line
- 应用范围：
Intracellular flow cytometry
- 浓度：
1:11
- 靶点：
c-Cbl pY700
- 抗体英文名：
c-Cbl pY700 Antibody, anti-human/mouse, REAfinity™
- 抗体名：
c-Cbl pY700 Antibody, anti-human/mouse, REAfinity™
- 规格：
100 tests in 1 mL
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文献和实验Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
for making working standards; store reconstituted standard at −20°C Mouse osteocalcin antiserum (biotinylated osteocalcin, antibody specific for the C‐terminus), one 12‐ml vial
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
in 100µl of elution buffer at 65°C for 5 minutes;7.The eluted complexes can then be electrophoresed in denaturing PAGE and subjected to western blot analysis with an anti-Flag antibody for detection of the particular flag-tagged protein of choice (Figure
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