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Isotype Control Antibody, rat

IgG2b, VioGreen™, 30 µg in 1 mL
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  • 询价
  • Miltenyi Biotec已认证
  • 德国
  • 130-102-659
  • 2025年12月09日
  • Flow cytometry, Functional assay
  • rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      KLH

    • 亚型

      rat IgG2bκ

    • 形态

      Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.

    • 保存条件

      避光,2-8℃

    • 克隆性

      ES26-5E12.4

    • 标记物

      VioGreen

    • 保质期

      24个月

    • 供应商

      Miltenyi Biotec

    • 宿主

      rat

    • 应用范围

      Flow cytometry, Functional assay

    • 浓度

      1:10

    • 靶点

      KLH

    • 抗体英文名

      Isotype Control Antibody, rat IgG2b

    • 抗体名

      Isotype Control Antibody, rat IgG2b

    • 规格

      30 µg in 1 mL

    Isotype control antibody for flow cytometric analysis of cellsThe Rat IgG2b isotype control antibody clone ES26-5E12.4 is specific for KLH (keyhole limpet hemocyanin). This protein is not expressed on mammalian cells or cell lines. Therefore, the antibody clone ES26-5E12.4 can be used as a negative control to distinguish specific from non-specific binding of Rat IgG2b fluorochrome-conjugated antibodies to human and mouse cells, for example, via Fc receptors, or due to interactions of the fluorochrome with the cell surface.

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    图标文献和实验
    相关实验
    • Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas

      are very stable - even in long term large scale cultures (bioreactors). b) High levels of monoclonal antibody secretion in culture - often 100 micrograms per ml of ordinary tissue culture, without recloning, and up to 5 milligrams per ml in hollow fibre

    • Immunofluorescent Staining of Mouse and Rat Leukocytes

      secondary reagent (e.g., fluorochrome-conjugated avidin, streptavidin, anti-Ig allotype, anti-Ig isotype, polyclonal anti-Ig). For example, dilute antibody to ~1 µg per 100 µl in wash buffer and add this to each well containing the loosened cell pellet

    • AbC Anti-Rat/Hamster Bead Kit

      capture beads (Component A) and negative beads (Component B) by gently vortexing for 30 seconds before use. 2)      Label a sample tube for each fluorochrome-conjugated rat or hamster antibody you are using, and add 1 drop of AbC RH capture beads

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