- 询价
- Miltenyi Biotec
- 德国
- 130-108-390
- 2025年12月09日
- Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry
- hamster
- mouse, rat, non-human primate
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
CD81
- 亚型：
hamster IgGκ
- 形态：
Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
- 保存条件：
避光，2-8℃
- 克隆性：
EAT2
- 标记物：
VioGreen
- 适应物种：
mouse, rat, non-human primate
- 保质期：
24个月
- 供应商：
Miltenyi Biotec
- 宿主：
hamster
- 应用范围：
Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry
- 浓度：
1:10
- 靶点：
CD81
- 抗体英文名：
CD81 Antibody, anti-mouse/rat
- 抗体名：
CD81 Antibody, anti-mouse/rat
- 规格：
9 µg in 300 µL
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文献和实验Immunofluorescent Staining of Mouse and Rat Leukocytes
16 (Mouse BD Fc Block™; Cat No. 553141/553142) or anti-rat CD32 (Rat BD Fc Block™; Cat. No. 550270/550271) is recommended. BD Fc Block™ can be added to cells (~0.25 µg per million cells, 3 - 5 min, 4°C) and need not be washed out prior to addition of primary
the 3 hour incubation Dynabeads™ M-280 Streptaviden magnetic beads (7x107 beads, ~100µl/sample) are added to the respective samples (note 1);5.The resultant siRNA/Flag complexes are then eluted with magnetic bead binding, the solution can be discarded
Phycoerythrin conjugation protocol
1 ml purified antibody at approx 2.5 mg/ml in PBS. Antibody must be fairly pure - i.e. purify on protein A or G column before conjugating. Add 20 µl SMCC, 1.7 mg/ml in DMSO. Incubate for 1 hour. Take product from step (2) and add
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