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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
CD2
- 亚型:
mouse IgG2bκ
- 形态:
Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
- 保存条件:
避光,2-8℃
- 克隆性:
LT2
- 标记物:
Vio Bright B515
- 适应物种:
human, non-human primate
- 保质期:
24个月
- 供应商:
Miltenyi Biotec
- 宿主:
mouse
- 应用范围:
Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry, Functional assay
- 浓度:
1:50
- 靶点:
CD2
- 抗体英文名:
CD2 Antibody, anti-human
- 抗体名:
CD2 Antibody, anti-human
- 规格:
100 tests in 200 µL
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文献和实验Isolation of Untouched Human B Cells by Depletion of Non-B Cells
in the same volume of Isolation Buffer as the initial volume of Dynabeads ®(step 2). 2. Isolation Procedure 1) Transfer 500 μl (5 × 107 ) PBMC in Isolation Buffer to a tube. 2) Add 100 μl of Antibody Mix. 3) Mix
In a 2 ml round-bottomed microcentrifuge tube mix 100 μl anti-coagulated blood with 1 ml pre-diluted Red Cell Lysis Buffer (diluted 1:5 from the original concentration supplied, stored at 2-8°C and used cold). If less than 10 μl of blood
1 μL 5) Label another sample tube for each fluorochrome-conjugated antibody you are using, and add 1 drop of AbC™ capture beads (Component A in the AbC™ Anti-Mouse Bead Kit) to each tube. 6)
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