CD180 (RP105) Antibody, anti-m

Identification and enumeration of CD180 (RP105)+ cells by flow cytometryClone RP/14 recognizes CD180, a type I transmembrane protein structurally similar to Toll like receptors (TLRs), with the conserved features of TLRs, including a leucine-rich repeat extracellular domain and regions containing conserved cysteines. However, in contrast to TLRs, CD180 lacks a typical TIR domain, containing only 6 to 11 intracytoplasmic amino acids. Antibody mediated ligation of CD180 was shown to induce B cell proliferation and protection from subsequent radiation- or dexamethasone-induced apoptosis. Expression of CD180 is found on mature B cells, monocytes, macrophages, and dendritic cells. CD180 activity is dependent on physical association with a molecule called MD-1. In B cells, CD180, together with TLR4, has been shown to induce B cell proliferation in response to LPS. However, in primary myeloid cells and in vivo, CD180 acts as an inhibitor of TLR4 signaling.