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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
CD154
- 亚型:
hamster IgG3κ
- 形态:
Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
- 保存条件:
避光,2-8℃
- 克隆性:
MR1
- 标记物:
Biotin
- 适应物种:
mouse
- 保质期:
24个月
- 供应商:
Miltenyi Biotec
- 宿主:
hamster
- 应用范围:
Flow cytometry, Intracellular flow cytometry
- 浓度:
1:10
- 靶点:
CD154
- 抗体英文名:
CD154 Antibody, anti-mouse
- 抗体名:
CD154 Antibody, anti-mouse
- 规格:
30 µg in 1 mL
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文献和实验分别利用 CD3 与 CD28 的功能学抗体,以及 CD3/CD28 Streptamer® 激活扩增 T 细胞的实验步骤与实验结果。 CD3/CD28抗体法 1、实验步骤: 1.1 抗体包被 用无菌 PBS 将 anti-mouse CD3 抗体(克隆号:145-2C11)稀释至 5μg/ml,稀释后的抗体加入到 24 孔板中,每孔 400μl,4°C 包被过夜。(注:板子在加入抗体之前,先用无菌 PBS 润洗 2-3 遍,避免干孔); 1.2 小鼠脾脏淋巴细胞分离(次日) 1. 将 70μm
实验步骤 1. Using the AbC™ Anti-Mouse Bead Kit 1) Completely resuspend the AbC™ capture beads (Component A) and negative beads (Component B) by gently vortexing for 30 seconds before use. 2) Label
MPG (3µl/ml of media described in (Morris et al. 2004)(note 3). .Pulldown (1), Antibody specific Forty-eight hours following the biotin linked sense or antisense siRNA transfection the cultures are collected and a chromatin immunoprecipitation (ChIP
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