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LGR5 Antibody, anti-mouse, APC

-Vio® 770, 30 µg in 200 µL
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  • 询价
  • Miltenyi Biotec已认证
  • 德国
  • 130-111-392
  • 2026年01月24日
  • Flow cytometry
  • rat
  • mouse
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      LGR5

    • 亚型

      rat IgG2b

    • 形态

      Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.

    • 保存条件

      避光,2-8℃

    • 克隆性

      DA04-10E8.9

    • 标记物

      APC-Vio 770

    • 适应物种

      mouse

    • 保质期

      24个月

    • 供应商

      Miltenyi Biotec

    • 宿主

      rat

    • 应用范围

      Flow cytometry

    • 浓度

      1:50

    • 靶点

      LGR5

    • 抗体英文名

      LGR5 Antibody, anti-mouse

    • 抗体名

      LGR5 Antibody, anti-mouse

    • 规格

      30 µg in 200 µL

    Identification and enumeration of LGR5+ cells by flow cytometryClone DA04-10E8.9 recognizes the mouse LGR5 antigen, also known as leucine-rich-repeat-containing G-protein–coupled receptor 5 or Gpr49 but not with its close homologues LGR4 or LGR6. LGR5 associates with Wnt-receptors and act as R-spondin receptor thereby playing a central role in the modulation of Wnt/β-catenin signaling in normal and neoplastic stem cells. Initially described as a highly specific marker for stem cells in the small intestine, colon, hair follicle, stomach, and during kidney development, LGR5 positive cells were also shown to be crucial during the development and progression of cancer. It was shown that LGR5 positive crypt stem cells are the cells-of-origin in intestinal cancer and that CSCs in human colorectal cancer can be identified and isolated based on LGR5 expression. |

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    图标文献和实验
    相关实验
    • AbC Anti-Mouse Bead Kit

      实验步骤   1.  Using the AbC™ Anti-Mouse Bead Kit 1)        Completely resuspend the AbC™ capture beads (Component A) and negative beads (Component B) by gently vortexing for 30 seconds before use. 2)        Label

    • ANTIBODY BINDING TO PROTEIN A AND PROTEIN G

      of various immunoglobulines. Protein A is a 42 kDa polypeptide that binds the residues in the CH2 and CH3 domains of immunoglobulin heavy chains. Protein G is an approximatley 30 kDa polypeptide that binds immunoglobulins and albumin. Recombinant protein G

    • Monoclonal Antibody Production

      drip off the end of the tail until you have collected ~200 µl.(You may have to pre-bleed twice,with a week or so between bleeds). (5) Take the collected blood and place at 37 ℃ for 30 min.to remove complement. (6) Place blood at 4 ℃ overnight to clot

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