Mouse anti-CD45RO Monoclonal A

产品描述：CD45 is a protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. CD45 acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, CD45 recruits and dephosphorylates SKAP1 and FYN

别名:L-CA; protein tyrosine phosphatase, receptor type, c polypeptide; T200 leukocyte common antigen; T200 glycoprotein; receptor-type tyrosine-protein phosphatase C; LY5; Leukocyte common antigen; GP180; CD45R; B220; CD45 antigen; LCA; CD45; CD antigen CD45; T200

免疫原：Cultured T-cells from an IL-2-dependent T-cell line

克隆性:UCHL-1

应用稀释比例：Flow-Cyt Live or cells fixed in 4% formaldehyde and permeabilized with 90% methanol. 5 µl per 1 x 10^6 cells. Immunocytochemistry (ICC) 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. Immunohistochemistry (IHC) 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. IHC-IF 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.. Multiplex Immunofluorescence (mIF) 1:250. Western Blot (WB) 1:250

应用注释：All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight

纯度:The antibody was purified from cell culture supernatant

防腐剂:Phosphate Buffered Saline (PBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide

保存说明:2 - 8°C

NCBI:NP_002829.3

UniProt ID:P08575

Note:For research use only.