- ￥2210
- biorbyt
- orb1499364
- 英国
- 2025年12月08日
- FC, WB
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 技术资料
- 抗体名：
BMAL1 Recombinant Rabbit Monoclonal Antibody抗体
- 抗体英文名：
BMAL1 Recombinant Rabbit Monoclonal Antibody
- 靶点：
BMAL1
- 浓度：
1mg/ml
- 应用范围：
FC, WB
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb1499364
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
Unconjugated
- 克隆性：
Recombinant
- 保存条件：
参考说明书
- 形态：
Liquid
- 亚型：
IgG
- 免疫原：
KLH conjugated synthetic peptide derived from human BMAL1
- 规格：
25 ul
产品描述：BMAL1 Recombinant Rabbit Monoclonal Antibody
别名:BMAL1_HUMAN; Basic helix-loop-helix ARNT-like protein 1; Aryl hydrocarbon receptor nuclear translocator-like protein 1; Basic-helix-loop-helix-PAS protein MOP3; Brain and muscle ARNT-like 1; Class E basic helix-loop-helix protein 5(bHLHe5); Member of PAS protein 3; PAS domain-containing protein 3; bHLH-PAS protein JAP3; ARNTL; BHLHE5; MOP3; PASD3; TIC; ARNTL1; BMAL1c;
免疫原：KLH conjugated synthetic peptide derived from human BMAL1
预测反应性:Rat
克隆性:8D1
分子量:69 kDa
应用稀释比例：WB=1:500-2000, Flow-Cyt=1:50-100
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:O00327
Note:For research use only.
别名:BMAL1_HUMAN; Basic helix-loop-helix ARNT-like protein 1; Aryl hydrocarbon receptor nuclear translocator-like protein 1; Basic-helix-loop-helix-PAS protein MOP3; Brain and muscle ARNT-like 1; Class E basic helix-loop-helix protein 5(bHLHe5); Member of PAS protein 3; PAS domain-containing protein 3; bHLH-PAS protein JAP3; ARNTL; BHLHE5; MOP3; PASD3; TIC; ARNTL1; BMAL1c;
免疫原：KLH conjugated synthetic peptide derived from human BMAL1
预测反应性:Rat
克隆性:8D1
分子量:69 kDa
应用稀释比例：WB=1:500-2000, Flow-Cyt=1:50-100
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:O00327
Note:For research use only.
Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: PC-3, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 68 kDa, Observed MW: 75 kDa.
Cell line: SH-SY5Y, Fixation: 4% Fixative, Permeabilization: 90% Methanol, Primary Ab Dilution: 1:100, Secondary Ab: Goat Anti-Rabbit IgG, Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line). Isotype control: Rabbit monoclonal IgG (Blue line). Comment: Line red is the positive signal for orb1499364.
ICC staining of BMAL1 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (orb1499364, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1000 dilution. The nuclear counter stain is DAPI (blue).
Tissue: Mouse hippocampus, Section type: Formalin fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1499364.
Tissue: Mouse pancreas, Section type: Formalin fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1499364.
Western blot analysis of BMAL1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (orb1499364, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate, Lane 2: NIH/3T3 cell lysate, Lane 3: Rat brain tissue lysate, Lane 4: Mouse spleen tissue lysate.
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BMAL1 Recombinant Rabbit Monoclonal Antibody抗体，orb1499364，biorbyt
￥2210
